不同浓度钛微粒对护骨素/护骨素配体基因表达影响的体外研究Effect of different Doses of titanium on gene expression of osteoprotegerin/osteoprotegerin ligand in MG-63 osteoblast-like cells
蔡贤华,陈安民,石晓兵
摘要(Abstract)：
目的: 探讨磨损微粒引起假体周围骨溶解的机理, 为人工关节假体松动的预防研究打下基础。方法: 采用不同浓度钛微粒 (粒径 <3μm) 对成骨细胞MG 63细胞进行 24h干预, 半定量RT PCR观察其对护骨素 (OPG) 及护骨素配体 (OPG- L) 基因表达的影响。结果:钛微粒能上调MG 63细胞OPG及OPG- L基因表达, 且对后者作用强于前者。结论: OPG- L/OPG比率随着钛微粒浓度的加大而增高, 引起骨溶解大于骨形成, 这可能是磨损微粒引起人工关节假体松动的重要原因, 这表明降低OPG- L/OPG比率有可能预防或逆转此病理过程。
关键词(KeyWords)： 钛微粒;护骨素;护骨素配体;人工关节
基金项目(Foundation):
作者(Author): 蔡贤华,陈安民,石晓兵
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DOI:
参考文献(References)：
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