陈淑强;徐又佳;俞晨;郝彦明;张振东;顾永平;

• 1:苏州大学附属第二医院骨科
• 2:苏州大学基础医学系病理教研室

摘要(Abstract)：

[目的]在大鼠急性脊髓损伤后不同时间硬膜外腔注射促红细胞生成素,观察并探讨其对脊髓神经功能的保护作用以及对脊髓神经细胞凋亡的影响。[方法]将48只体重(270±10)g成年雄性Sprague-Dawley大鼠随机分为正常组(n=6),假手术组(仅切除椎板,n=6),对照组(1、6、24h组分别给与等量生理盐水硬膜外注射,n=18),实验组(1、6、24h组分别给与5000u/kg-bw的rhEPO硬膜外注射,n=18)。按改良Allen打击法建立大鼠脊髓不完全损伤模型,通过动物神经运动功能BBB评分及斜板试验评价神经损伤程度;分别采用TUNEL法及免疫组化法检测脊髓神经细胞凋亡指数和Caspase-3,fas-fasl死亡蛋白的表达。光镜下观察统计分析结果。[结果]与对照组相比,脊髓损伤后促红细胞生成素1、6、24h干预的实验组神经运动功能均有改善,脊髓神经细胞凋亡指数均明显下降(P<0.01);实验组中,fas-fasl死亡蛋白较对照组表达减少。2组中神经细胞凋亡数与Caspase-3、fas-fasl死亡蛋白表达细胞数呈线性正相关(P<0.01),相关系数分别为r=0.941(Caspase-3)和r=0.929(fas-fasl)。[结论]早期应用外源性EPO对脊髓不完全损伤具有保护作用,可明显改善神经运动功能恢复情况,此种保护作用可能与EPO能够阻断Caspase-3、fas-fasl死亡蛋白途径的神经细胞凋亡有关。

关键词(KeyWords)： 脊髓损伤;促红细胞生成素;凋亡;Caspase-3;FasL;硬膜外腔注射

Abstract:

Keywords:

基金项目(Foundation): 江苏省卫生厅医学医学科研项目基金(编号:H200718);; 苏州市社会发展项目基金(编号ssy0625)

作者(Author): 陈淑强;徐又佳;俞晨;郝彦明;张振东;顾永平;

Email:

DOI:

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