舒鹏;程耀锋;毛瑞琪;文涔锦;孙登龙;席姣娅;叶树楠;

• 1:华中科技大学同济医学院附属协和医院骨科
• 2:华中科技大学同济医学院基础医学院生理学系

摘要(Abstract)：

股骨头坏死作为一种骨科常见疾病,拥有极高的致残率,且其发病逐年呈年轻化趋势,严重影响患者的生活质量。因此,早期防治股骨头坏死显得格外重要。外泌体是由细胞分泌的直径约30-100nm的囊泡状物,内含脂质、蛋白及核酸等,能调节局部微环境,促进组织损伤修复。本文就外泌体的形成、分泌以及不同来源外泌体在早期股骨头坏死防治的研究进展作一综述。

关键词(KeyWords)： 外泌体;股骨头坏死;干细胞;治疗

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目（编号：81472083）;; 湖北省自然科学基金项目（编号：2018CFB460）;; 华中科技大学自主创新研究基金项目（编号：2017KFYXJJ058）

作者(Author): 舒鹏;程耀锋;毛瑞琪;文涔锦;孙登龙;席姣娅;叶树楠;

Email:

DOI:

参考文献(References)：

• [1]房磊,魏开斌.股骨头坏死保头手术治疗进展[J].泰山医学院学报, 2018, 39(2):237-240.
• [2] Burke J, Kolhe R, Hunter M, et al. Stem cell-derived exosomes:a potential alternative therapeutic agent in orthopaedics[J]. Stem Cells Int, 2016, 2016(3):5802529.
• [3] Manca S, Upadhyaya B, Mutai E, et al. Milk exosomes are bioavailable and distinct microRNA cargos have unique tissue distribution patterns[J]. Sci Rep, 2018, 8(1):11321.
• [4] Herberts CA, Kwa MS, Hermsen HP. Risk factors in the development of stem cell therapy[J]. J Transl Med, 2011, 9(1):29.
• [5] Yu B, Zhang X, Li X. Exosomes derived from mesenchymal stem cells[J]. Int J Mol Sci, 2014, 15(3):4142-4157.
• [6] Arntz OJ, Pieters BC, Oliveira MC, et al. Oral administration of bovine milk derived extracellular vesicles attenuates arthritis in two mouse models[J]. Mol Nutr Food Res, 2015, 59(9):1701-1712.
• [7] Johnstone RM, Adam M, Hammond JR, et al. Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles(exosomes)[J]. J Biol Chem,1987, 262(19):9412-9420.
• [8] Van Niel G, D'angelo G, Raposo G. Shedding light on the cell biology of extracellular vesicles[J]. Nat Rev Mol Cell Biol, 2018, 19(4):213-228.
• [9] Jahn R, Scheller RH. SNAREs--engines for membrane fusion[J]. Nat Rev Mol Cell Biol, 2006, 7(9):631-643.
• [10] Colombo M, Raposo G, Thery C. Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles[J]. Annu Rev Cell Dev Biol, 2014, 30(1):255-289.
• [11] Fernandes G, Yang S. Application of platelet-rich plasma with stem cells in bone and periodontal tissue engineering[J]. Bone Res, 2016, 4:16036.
• [12] Tong S, Yin J, Liu J. Platelet-rich plasma has beneficial effects in mice with osteonecrosis of the femoral head by promoting angiogenesis[J]. Exp Ther Med, 2018, 15(2):1781-1788.
• [13] Guadilla J, Fiz N, Andia I, et al. Arthroscopic management and platelet-rich plasma therapy for avascular necrosis of the hip[J].Knee Surg Sports Traumatol Arthrosc, 2012, 20(2):393-398.
• [14] Torreggiani E, Perut F, Roncuzzi L, et al. Exosomes:novel effectors of human platelet lysate activity[J]. Eur Cell Mater, 2014, 28(1):137-151.
• [15] Tao SC, Yuan T, Rui BY, et al. Exosomes derived from human platelet-rich plasma prevent apoptosis induced by glucocorticoidassociated endoplasmic reticulum stress in rat osteonecrosis of the femoral head via the Akt/Bad/Bcl-2 signal pathway[J]. Theranostics, 2017, 7(3):733-750.
• [16] Grayson WL, Bunnell BA, Martin E, et al. Stromal cells and stem cells in clinical bone regeneration[J]. Nat Rev Endocrinol, 2015,11(3):140-150.
• [17] Ratajczak MZ, Jadczyk T, Pedziwiatr D, et al. New advances in stem cell research:practical implications for regenerative medicine[J]. Pol Arch Med Wewn, 2014, 124(7-8):417-426.
• [18] Tao SC, Yuan T, Zhang YL, et al. Exosomes derived from miR-140-5p-overexpressing human synovial mesenchymal stem cells enhance cartilage tissue regeneration and prevent osteoarthritis of the knee in a rat model[J]. Theranostics, 2017, 7(1):180-195.
• [19] Guo SC, Tao SC, Yin WJ, et al. Exosomes from human synovial-derived mesenchymal stem cells prevent glucocorticoid-induced osteonecrosis of the femoral head in the rat[J]. Int J Biol Sci, 2016,12(10):1262-1272.
• [20] Zhao P, Xiao L, Peng J, et al. Exosomes derived from bone marrow mesenchymal stem cells improve osteoporosis through promoting osteoblast proliferation via MAPK pathway[J]. Eur Rev Med Pharmacol Sci, 2018, 22(12):3962-3970.
• [21] Cosenza S, Toupet K, Maumus M, et al. Mesenchymal stem cellsderived exosomes are more immunosuppressive than microparticles in inflammatory arthritis[J]. Heranostics, 2018, 8(5):1399-1410.
• [22] Li H, Liu D, Li C, et al. Exosomes secreted from mutant-HIF-1alpha-modified bone-marrow-derived mesenchymal stem cells attenuate early steroid-induced avascular necrosis of femoral head in rabbit[J]. Cell Biol Int, 2017, 41(12):1379-1390.
• [23]周山健,李海乐,肖大伟,等. miR-126修饰的间质干细胞来源的外泌体对大鼠早期缺血性股骨头坏死的影响[J].西安交通大学学报(医学版), 2018, 39(3):379-385.
• [24] Qi X, Zhang J, Yuan H, et al. Exosomes secreted by human-induced pluripotent stem cell-derived mesenchymal stem cells repair critical-sized bone defects through enhanced angiogenesis and osteogenesis in osteoporotic rats[J]. Int J Biol Sci, 2016, 12(7):836-849.
• [25] Liu X, Li Q, Niu X, et al. Exosomes secreted from human-induced pluripotent stem cell-derived mesenchymal stem cells prevent osteonecrosis of the femoral head by promoting angiogenesis[J]. Int J Biol Sci, 2017, 13(2):232-244.
• [26] Mak J, Jablonski CL, Leonard CA, et al. Intra-articular injection of synovial mesenchymal stem cells improves cartilage repair in a mouse injury model[J]. Sci Rep, 2016, 6:23076.
• [27] Sekiya I, Muneta T, Horie M, et al. Arthroscopic transplantation of synovial stem cells improves clinical outcomes in knees with cartilage defects[J]. Clin Orthop, 2015, 473(7):2316-2326.
• [28] Zhu Y, Wang Y, Zhao B, et al. Comparison of exosomes secreted by induced pluripotent stem cell-derived mesenchymal stem cells and synovial membrane-derived mesenchymal stem cells for the treatment of osteoarthritis[J]. Stem Cell Res Ther, 2017, 8(1):64.
• [29] Xu S, Zhang L, Jin H, et al. Autologous stem cells combined core decompression for treatment of avascular necrosis of the femoral head:a systematic meta-analysis[J]. Biomed Res Int, 2017, 2017:6136205.
• [30] Tong PJ, Ye FS, Zhang SX, et al. Treatment of non-traumatic femoral head avascular necrosis by perfusion of bone marrow stromal stem cells through optional artery[J]. Zhongguo Gu Shang, 2014,27(7):565-569.
• [31] Calori GM, Mazza E, Colombo M, et al. Treatment of AVN using the induction chamber technique and a biological-based approach:indications and clinical results[J]. Injury, 2014, 45(2):369-373.
• [32] Zhao C, Ikeya M. Generation and applications of induced pluripotent stem cell-derived mesenchymal stem cells[J]. Stem Cells Int,2018, 2018:9601623.