黄霸;苏永蔚;高艺萌;罗华;刘丹平;綦惠;

• 1:锦州医科大学附属第一医院运动与关节科
• 2:锦州医科大学医疗学院
• 3:锦州医科大学附属奉贤医院骨科
• 4:北京市创伤骨科研究所

摘要(Abstract)：

[目的]观察从腺病毒-骨形态发生蛋白-2-绿色荧光蛋白(Ad-BMP-2-GFP)转染骨髓间充质干细胞(BMSCs)获取的含有BMP-2的上清对软骨细胞体外凋亡和体内再生的影响。[方法]通过Ad-BMP-2-GFP转染BMSCs使BMP-2高表达,采用冰冻干燥法浓缩BMSCs细胞上清至10倍浓度,过滤后-20℃储存备用。体外实验:加入IL-1β(18 ng/ml)建立软骨细胞体外炎症环境,施加转染后和未转染浓缩上清,并以PBS处理为空白对照组,流式细胞术检测各处理组软骨细胞凋亡水平。体内实验:20只雄性新西兰大白兔随机分为4组,除假手术组外,其他3组建立兔膝关节软骨缺损模型,分别注射等量的纯生理盐水、转染组和未转染的培养上清。6周后取材,通过大体标本和番红O固绿染色观察关节软骨缺损修复效果。[结果]体外实验显示,转染上清组的软骨细胞凋亡水平较未转染上清组和空白对照组明显减弱(P<0.05);体内实验显示,转染上清组对软骨缺损修复效果优于未转染上清组和单纯生理盐水组。[结论]通过Ad-BMP-2-GFP转染BMSCs,获取含有BMP-2的上清可以抑制体外炎症环境下软骨细胞凋亡,促进体内软骨缺损修复。

关键词(KeyWords)： 间充质干细胞;BMP-2;基因转染;培养上清;细胞凋亡;再生

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(编号:81572140);; 北京市自然科学基金面上项目(编号:7172036)

作者(Author): 黄霸;苏永蔚;高艺萌;罗华;刘丹平;綦惠;

Email:

DOI:

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