陆继鹏;舒钧;柏涛;熊鹰;浦波;李伟强;

• 1:昆明医学院附属延安医院骨科
• 2:昆明医学院第二附属医院骨科
• 3:云南省玉溪市人民医院骨科

摘要(Abstract)：

[目的]通过增加软骨下骨硬度模拟软骨下骨硬化,诱发骨关节炎的发生,探讨骨关节炎发病机制。[方法]调整聚甲基丙烯酸甲酯粉剂(polymethylmethacrylate,PMMA)、甲基丙烯酸甲酯液剂(methylmethacrylate,MMA)、羟基磷灰石(hydroxyapatite,HA)和蒸馏水的比例,使反应温度低于40℃,测量该比例下聚合后的极限强度和刚度并与软骨下骨比较,制得PMMA/HA复合材料。刮除兔胫骨平台内侧软骨下骨后置入复合材料,对术后3、6、9、12周的关节软骨进行组织学观察,免疫组化法检测Ⅱ型胶原和基质金属蛋白酶-1(matrix metallo proteinase-1,MMP-1)在软骨中的表达和分布,并与空白、对照组比较。透射电镜观察空白、6、12周组软骨细胞改变。[结果]该复合材料置入软骨下骨后,随观察时间延长实验组逐渐出现退变,Mankin分级逐渐升高,电镜也显示实验组软骨细胞退变表现。免疫组化显示Ⅱ型胶原表达增加主要在移行层和深层上部,MMP-1表达以软骨表层及中上层居多,随观察时间延长二者染色强度均逐渐升高。[结论]增加软骨下骨硬度后,诱发了兔膝骨关节炎。提示软骨下骨硬化可引起软骨退变,其可能是骨关节炎的病因之一。

关键词(KeyWords)： 关节软骨;软骨下骨硬化;骨关节炎;免疫组化;兔

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 陆继鹏;舒钧;柏涛;熊鹰;浦波;李伟强;

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