张宏其;郭强;郭超峰;唐明星;高琪乐;尹新华;吴建煌;刘金洋;

• 1:中南大学湘雅医院脊柱外科湘雅医院脊柱外科中心

摘要(Abstract)：

[目的]分析硫酸化相关酶在AIS患者关节突软骨中的表达特点,为软骨细胞的力学研究和延缓脊柱侧凸进展提供参考。[方法]选取18例Lenke 1型AIS患者顶椎和下端椎凹、凸侧和对照组(15例)的下关节突软骨作为研究对象,固定脱钙后制成石蜡切片。免疫组化染色测定分析硫酸化反应相关酶PAPSS2、PAPST1和CHST1在Lenke 1型AIS患者顶椎和下端椎关节突软骨中的表达,利用Leica显微镜对免疫组化图像进行观察,用Image-pro Plus软件将每个视野中的着色面积、光密度以及目标数目转换成累积光密度值(IOD),代表相应酶的表达强度,最后进行统计学分析。[结果]PAPSS2主要分布在软骨细胞质和软骨囊基质;PAPST1特异性分布在软骨细胞质;CHST15主要分布在软骨细胞质和软骨囊基质。硫酸化反应相关酶PAPSS2、PAPST1和CHST15的表达转换成IOD有以下分布特点:对照组两侧的表达差异无统计学意义;顶椎凹侧高于凸侧,顶椎凸侧高于对照组,差异有统计学意义;顶椎高于同侧下端椎,差异有统计学意义;下端椎的凹凸侧的表达差异无统计学意义;下端椎的表达与对照组的表达比较差异无统计学意义。[结论]青少年特发性脊柱侧凸顶椎关节突硫酸化反应相关酶的表达增强与特发性脊柱侧凸进展相关。

关键词(KeyWords)： 青少年特发性脊柱侧凸;关节突软骨;硫酸化相关酶

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 张宏其;郭强;郭超峰;唐明星;高琪乐;尹新华;吴建煌;刘金洋;

Email:

DOI:

参考文献(References)：

• [1]程永红.青少年特发性脊柱侧凸治疗研究进展[J].中国矫形外科杂志,2012,20(9):819-822.
• [2]Roth AK,Bogie R,Jacobs E,et al.Large animal models in fusionless scoliosis correction research:a literature review[J].Spine J,2013,13(6):675-688.
• [3]Walsh G,Jefferis R.Post-translational modifications in the context of therapeutic proteins[J].Nat Biotechnol,2006,24(10):1241-1252.
• [4]Zhang HQ,Lu SJ,Tang MX,et al.Association of estrogen receptor beta gene polymorphisms with susceptibility to adolescent idiopathic scoliosis[J].Spine(Phila Pa 1976),2009,34(8):760-764.
• [5]Bornes TD,Jomha NM,Mulet-Sierra A,et al.Hypoxic culture of bone marrow-derived mesenchymal stromal stem cells differentially enhances in vitro chondrogenesis within cell-seeded collagen and hyaluronic acid porous scaffolds[J].Stem Cell Res Ther,2015,6(1):84.
• [6]Mertz EL,Facchini M,Pham AT,et al.Matrix disruptions,growth,and degradation of cartilage with impaired sulfation[J].J Biol Chem,2012,287(26):22030-22042.
• [7]Cao J,Li S,Shi Z,et al.Articular cartilage metabolism in patients with Kashin-Beck Disease:an endemic osteoarthropathy in China[J].Osteoarthritis Cartilage,2008,16(6):680-688.
• [8]Sekulic N,Dietrich K,Paarmann I,et al.Elucidation of the active conformation of the APS-kinase domain of human PAPS synthetase1[J].J Mol Biol,2007,367(2):488-500.
• [9]Henry SP,Liang S,Akdemir KC,et al.The postnatal role of Sox9 in cartilage[J].J Bone Miner Res,2012,27(12):2511-2525.
• [10]邱贵兴,李其一,刘勇,等,青少年特发性脊柱侧凸患者关节突软骨中转化生长因子-β1及碱性成纤维细胞生长因子表达的研究[J].中华医学杂志,2006,86(21):1478-1483.
• [11]Ramaswamy G,Sohn P,Eberhardt A,et al.Altered responsiveness to TGF-beta results in reduced Papss2 expression and alterations in the biomechanical properties of mouse articular cartilage[J].Arthritis Res Ther,2012,14(2):49.