崔开;韩亚新;孔冉冉;董君博;张国栋;梁栋;屠冠军;

• 1:中国医科大学附属第一医院骨科

摘要(Abstract)：

[目的]研究大鼠急性脊髓损伤后Caspase-3、Cathepsin B的表达,并初步探讨Caspase-3、Cathepsin B在脊髓损伤后表达的意义。[方法]将78只成年健康SD大鼠按Nystrom法建立大鼠脊髓(T8、T9)急性压迫损伤模型,HE染色观察脊髓组织病理学变化,免疫组化测定各时间点Cathepsin B、Caspase-3的表达变化,原位末端脱氧核糖核酸转移酶介导的脱氧尿苷三磷酸(dUTP)标记法(TUNEL法)检测神经细胞的凋亡水平。[结果]免疫组化结果显示正常及假手术组大鼠脊髓神经细胞中Caspase-3,Cathepsin B,TUNEL阳性细胞较少;在脊髓损伤后3dCathepsin B阳性细胞数明显增多,5d达高峰,7d未见明显衰减。Caspase-3阳性细胞数在脊髓损伤后8h明显增多,3d达高峰,7d表达减弱。TUNEL阳性细胞数也在8h明显增多,3d达高峰,7d表达减弱。Cathepsin B阳性细胞形态及表达的部位均与Caspase-3阳性细胞、TUNEL阳性细胞差别较大。[结论]Caspase-3参与了脊髓损伤细胞凋亡的调节,

关键词(KeyWords)： 脊髓损伤;细胞凋亡;Cathepsin B;Caspase-3

Abstract:

Keywords:

基金项目(Foundation): 辽宁省教育厅基金资助项目(编号:2004C044)

作者(Author): 崔开;韩亚新;孔冉冉;董君博;张国栋;梁栋;屠冠军;

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DOI:

参考文献(References)：

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