郝春香;黄靖香;眭翔;韩春姬;陈国庆;张昌盛;郭全义;刘舒云;

• 1:中国人民解放军总医院麻醉科
• 2:中国人民解放军总医院骨科研究所北京市再生医学重点实验室/全军战创伤重点实验室

摘要(Abstract)：

[目的]以中国山羊为动物模型,观察藻酸钙复合自体软骨细胞修复膝关节负重区软骨缺损的可行性。[方法]取羊肩关节软骨,分离、培养软骨细胞,蕃红"O"、 Giemsa及Ⅱ型胶原免疫组织化学染色对其进行鉴定。将自体软骨细胞与藻酸钙凝胶复合,修复山羊股骨髁负重区全层软骨缺损(直径6 mm),实验分为四组:(1)缺损旷置组:缺损内未植入任何组织;(2)骨膜覆盖组:自体骨膜覆盖缺损区;(3)藻酸钙+骨膜组:凝胶植入软骨缺损区,并用自体骨膜覆盖;(4)藻酸钙+细胞+骨膜组:藻酸钙复合自体软骨细胞植入软骨缺损区,自体骨膜覆盖;分别于手术后3、6个月取材,通过大体观察及组织学评分检测修复效果。[结果]软骨细胞复合物蕃红"O"、 Giemsa染色及Ⅱ型胶原免疫组化染色结果均为阳性,将藻酸钙凝胶-软骨细胞复合物用于羊负重区关节面软骨缺损修复,从大体观察和组织学评分进行比较,发现各组均有不同程度的组织修复,藻酸钙+细胞+骨膜组效果最好,与其他组差异有统计学意义(P<0.05)。[结论]藻酸钙凝胶-软骨细胞复合物结合自体骨膜覆盖,可较好修复山羊膝关节负重区软骨缺损。

关键词(KeyWords)： 软骨;组织工程;再生

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划(编号:2017YFC1103404,2017YFC1104102);; 国家高技术研究发展计划(863计划)(编号:2012AA020502);; 国家自然科学基金项目(编号:81472092,81772319);; 北京市自然科学基金项目(编号:7172203);; 北京市科技专项(编号:Z161100005016059)

作者(Author): 郝春香;黄靖香;眭翔;韩春姬;陈国庆;张昌盛;郭全义;刘舒云;

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