张宏其;高琪乐;郭超峰;陈筱;郭虎兵;李劲松;赵迪;鲁世金;王瑶;

• 1:中南大学湘雅医院脊柱外科湘雅脊柱外科中心

摘要(Abstract)：

[目的]分析趋化因子单核细胞趋化蛋白-1(MCP-1)启动子区362基因型频率和等位基因多态性与中国湖南省汉族人群脊柱结核易感性的关系。[方法]选取2007年12月～2010年12月本院收治的湖南汉族新发脊柱结核患者166例(病例组)及健康志愿者200例(对照组),应用聚合酶链式反应(PCR)和DNA直接测序法检测两组患者MCP-1-362位点的多态性,并进行MCP-1基因分型。[结果]MCP-1-GG、GC、CC三种基因型在病例组和对照组中的分布频率分别为18.1%、42.8%、39.2%和25.5%、48.0%、26.5%。两组组间比较有显著性差异(P<0.05),其中MCP-1-CC基因型在病例组中的分布频率明显高于对照组,比值比(odds ratio,OR)为1.478,其95%可信区间(confidence interval,CI)为1.096～1.992。[结论]在湖南汉族人群中,MCP-1-362位点CC基因型可能与脊柱结核的易感性相关。

关键词(KeyWords)： 单核细胞趋化蛋白-1;基因多态性;脊柱结核

Abstract:

Keywords:

基金项目(Foundation): 中央高校基本科研业务费专项资金资助项目(编号:2012QNZT101)

作者(Author): 张宏其;高琪乐;郭超峰;陈筱;郭虎兵;李劲松;赵迪;鲁世金;王瑶;

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参考文献(References)：

• [1]全国结核病流行病学抽样调查技术指导组.第四次全国结核病流行病学抽样调查报告[J].中华结核和呼吸杂志,2002,1:6-10.
• [2]Schirmer P,Renault CA,Holodniy M.Is spinal tuberculosis contagious[J].Int J Infect Dis,2010,8:659-666.
• [3]Ozol D,Koktener A,Uyar ME.Active pulmonary tuberculosis with ver-tebra and rib involvement:case report[J].South Med J,2006,2:171-173.
• [4]Teo HE,Peh WC.Skeletal tuberculosis in children[J].Pediatr Radiol,2004,11:853-860.
• [5]Vatutina VV,Kovalenko KN.Tuberculosis of respiratory organs concom-itant with tuberculosis of bones and joints in young children[J].ProblTuberk Bolezn Legk,2003,5:31-33.
• [6]Kaya A,Topu Z,Fitoz S,et al.Pulmonary tuberculosis with multifo-cal skeletal involvement[J].Monaldi Arch Chest Dis,2004,2:133-135.
• [7]Schlesinger N,Lardizabal A,Rao J,et al.Tuberculosis of the spine:experience in an inner city hospital[J].J Clin Rheumatol,2005,1:17-20.
• [8]刘忠泉,宋长兴,胡秀龄,等.HLA-DR、DQ基因与骨关节结核的易感研究[J].中国防痨杂志,2000,3:130-133.
• [9]Bellamy R.Interferon-gamma and host susceptibility to tuberculosis[J].Am J Respir Crit Care Med,2003,7:946-947.
• [10]Awomoyi AA,Marchant A,Howson JM,et al.Interleukin-10,polymor-phism in SLCllA1(formerly NRAMPl),and susceptibility to tuberculo-sis[J].J Infect Dis,2002,186:1808-1814.
• [11]Roth DE,Solo G,Arenas F,et al.Association between vitamin D re-ceptor gene polymorphisms and response to treatment of pulmonary tu-berculosis[J].J Infect Dis,2004,190:920-927.
• [12]Seborg C,Madsen HO,Andersen AB,et al.Mannose-binding lectinpolymorphisms in clinical tuberculosis[J].J Infect Dis,2003,188:777-782.
• [13]Tso HW,Lau YL,Tam CM,et al.Associations between ILl2B poly-morphisms and tuberculosis in the Hong Kong Chinese population[J].J Infect Dis,2004,190:913-919.
• [14]Algood HM,Chan J,Flynn JL.Chemokines and tuberculosis[J].Cytokine Growth Factor Rev,2003,14:467-477.
• [15]Scott HM,Flynn JL.Mycobacterium tuberculosis in chemokine re-ceptor2-deficient mice:influence of dose on disease progression[J].Infect Immun,2002,70:5946-5954.
• [16]杨本付,庄斌,李芳,等.单核细胞趋化蛋白-1基因多态性与肺结核易感性的关系[J].中华结核和呼吸杂志,2009,6:454-456.
• [17]徐珍娥,解元元,陈军华,等.重庆汉族儿童单核细胞趋化蛋白1基因多态性及表达水平与结核病易感性的关系[J].中华儿科杂志,2009,3:200-203.
• [18]Thye T,Nejentsev S,Intemann CD,et al.MCP-1promoter variant-362C associated with protection from pulmonary tuberculosis in Ghana,West Africa[J].Hum Mol Genet,2009,2:381-388.
• [19]Jamieson SE,Miller EN,Black GF,et al.Evidence for a cluster of genes on chromosome17q11-q21controlling susceptibility to tuber-culosis and leprosy in Brazilians[J].Genes Immun,2004,5:46-57.
• [20]Velez EDR,Tacconelli A,Wejse C,et al.MCP1SNPs and pulmonary tuberculosis in cohorts from west Africa,the USA and Argentina:lack of association or epistasis with IL12B polymorphisms[J]PLOS ONE,2012,2:e32275.