干细胞在软骨组织工程中的研究现状及进展
徐佳元;杨卫良;
摘要(Abstract):
<正>干细胞(stem cells,SCs)是一类未充分分化的、具有自我复制能力的多潜能细胞,因其具有在一定条件下可以再生人体各种组织器官的潜在功能,被在肿瘤、遗传性疾病和组织器官损伤的治疗等方面寄予厚望。由创伤或非创伤性病理损害如类风湿性关节炎等所造成的关节软骨缺损在临床上十分常见,由于关节软骨的修复能力有限,软骨缺损的治疗存在巨大挑战。而胚胎干细胞(embryonic stem cells,ESCs)和间充质干细胞(mesenchymal stem cells,MSCs)具有自我更新和多功能分化潜能的特性,在适当培养条件下可被诱导分化
关键词(KeyWords): 干细胞;软骨组织工程;现状;进展
基金项目(Foundation):
作者(Author): 徐佳元;杨卫良;
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DOI:
参考文献(References):
- [1]Dominici M,Le Blanc K,Mueller I,et al.Minimal criteria for defi-ning multipotent mesenchymal stromal cells.The international society for cellular therapy position statement[J].Cytotherapy,2006,4:315-317.
- [2]Wagner W,Wein F,Seckinger A,et al.Comparative characteristics of mesenchymal stem cells from human bone marrow,adipose tissue,and umbilical cord blood[J].Exp Hematol,2005,11:1402-1416.
- [3]Yamamoto N,Akamatsu H,Hasegawa S.Isolation of multipotent stem cells from mouse adipose tissue[J].J Dermatol Sci,2007,1:43-52.
- [4]Jukes JM,Moroni L,van Blitterswijk CA,et al.Critical steps towarda tissue-engineered cartilage implant using embryonic stem cells[J].Tissue Eng Part A,2008,1:135-147.
- [5]Nakajima M,Wakitani S,Harada Y,et al.In vivo mechanical condi-tion plays an important role for appearance of cartilage tissue in ES cell transplanted joint[J].J Orthop Res,2008,1:10-17.
- [6]Bigdeli N,Karlsson C,Strehl R,et al.Coculture of human embryonic stem cells and human articular chondrocytes results in significantly al-tered phenotype and improved chondrogenic differentiation[J].Stem Cells,2009,27:1812-1821.
- [7]Wei A,Chung SA,Tao H,et al.Differentiation of rodent bone mar-row mesenchymal stem cells into intervertebral disc-like cells follow-ing coculture with rat disc tissue[J].Tissue Eng Part A,2009,15:2581-2595.
- [8]Mo XT,Guo SC,Xie HQ,et al.Variations in the ratios of co-cul-tured mesenchymal stem cells and chondrocytes regulate the expression of cartilaginous and osseous phenotype in alginate constructs[J].Bone,2009,45:42-51.
- [9]Toh WS,Yang Z,Liu H,et al.Effects of culture conditions and bone morphogenetic protein2on extent of chondrogenesis from human em-bryonic stem cells[J].Stem Cells,2007,4:950-960.
- [10]Bell DM,Leung KKH,Wheatley SC,et al.SOX9directly regulates the type-Ⅱcollagen gene[J].Nat Genet,1997,16:174-178.
- [11]de Crombrugghe B,Lefebvre V,Behringer R R,et al.Transcriptional mechanisms of chondrocyte differentiation[J].Matrix Biol,2000,19:389-394.
- [12]Hargus G,Kist R,Kramer J,et al.Loss of Sox9function results in defective chondrocyte differentiation of mouse embryonic stem cells in vitro[J].Int J Dev Biol,2008,4:323-332.
- [13]Ikeda T,Kamekura S,Mabuchi A,et al.The combination of SOX5,SOX6and SOX9(the SOX trio)provides signals sufficient for induc-tion of permanent cartilage[J].Arthritis Rheum,2004,50:3561-3573.
- [14]Majumdar MK,Wang E,Morris EA.BMP-2and BMP-9promotes chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1[J].J Cell Physiol,2001,189:275-284.
- [15]Derfoul A,Carlberg AL.Differential regulation of osteoggenic marker gene expression by Wnt-3a in embryonic mesenchymal multipotential progenitor cells[J].Differentiation,2004,72:209-223.
- [16]Tardif G,Reboul P,Dupuis M,et al.Transforming growth factor beta induced collagenase-3production in human osteoarthritic chondro-cytes is triggered by Smad proteins:cooperation between activator pro-tein-1and PEA-3binding sites[J].J Rheumatol,2001,28:1631-1639.
- [17]Smith N,Dong Y,Lian JB,et al.Overlapping expression of Runx1(Cbfa2)and Runx2(Cbfa1)transcription factors supports cooperative induction of skeletal development[J].J Cell Physiol,2005,203:133-143.
- [18]Greco SJ,Liu K,Rameshwar P.Functional similarities among genes regulated by OCT4in human mesenchymal and embryonic stem cells[J].Stem Cells,2007,12:3143-3154.
- [19]Zheng L,Fan HS,Sun J,et al.Chondrogenic differentiation of mes-enchymal stem cells induced by collagen-based hydrogel:an in vivo study[J].Biomed Mater Res A,2010,2:783-792.
- [20]王文良,张华亮,关静,等.壳聚糖/羟基磷灰石支架修复骨软骨缺损的实验研究[J].中国矫形外科杂志,2008,20:1579-1583.
- [21]Mrugala D,Bony C,Neves N,et al.Phenotypic and functional char-acterization of ovine mesenchymal stem cells:application to a cartilage defectmodel[J].Ann Rheum Dis,2008,3:288-295.
- [22]Seung HH,Yun HK,Min SP,et al.Histological and biomechanical properties of regenerated articular cartilage using chondrogenic bone marrow stromal cells with a PLGA scaffold in vivo[J].J Biomed Ma-ter Res,2008,4:850-861.
- [23]Wakitani S,Nawata M,Tensho K,et al.Repair of articular cartilage defects in the patello-femoral joint with autologous bone marrow mes-enchymal cell transplantation:three case reports involving9defects in 5knees[J].J Tissue Eng Regen Med,2007,1:74-79.
- [24]Kuroda R,Ishida K,Matsumoto T,et al.Treatment of a full-thick-ness articular cartilage defect in the femoral condyle of an athlete with autologous bone-marrow stromal cells[J].Osteoarthritis Cartilage,2007,2:226-231.
- [25]Tateishi K,He J,Taranova O,et al.Generation of insulin-secreting islet-like clusters from human skin fibroblasts[J].J Biol Chem,2008,46:31601-31607.
- [26]Schenke-Layland K,Rhodes KE,Angelis E,et al.Reprogrammed mouse fibroblasts differentiate into cells of the cardiovascular and hem-atopoietic lineages[J].Stem Cells,2008,6:1537-1546.
- [27]Soldner F,Hockemeyer D,Beard C,et al.Parkinson’s disease patient-derived induced pluripotent stem cells free of viral reprogramming factors[J].Cell,2009,5:964-977.