赵海燕;夏亚一;康鹏德;

• 1:兰州大学第二医院骨科研究所
• 2:四川大学华西医院骨科

摘要(Abstract)：

[目的]探讨应用新型材料掺锶聚磷酸钙(strontium-doped calcium polyphosphate,SCPP)修复兔激素性股骨头坏死骨缺损过程中血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)的表达及其意义。[方法]成年健康雄性日本大白兔21只,右侧臀肌注射甲泼尼龙20mg/kg,每日1次,共3次,建立兔激素性股骨头坏死动物模型。2周时随机处死3只动物取材组织学证实造模成功后,其余18只,通过髋关节后外侧入路活门法(Trap-door)清除骨坏死组织,制成双侧股骨头直径2.5mm×5mm的圆柱形骨缺损,随机分为3组。A组植入掺锶聚磷酸钙;B组植入聚磷酸钙:C组植入自体松质骨。术后4、8、12周分批处死各组动物,通过影像学、组织病理学、VEGF免疫组织化学染色观察评价股骨头坏死骨缺损修复过程中VEGF的表达及其意义。[结果]各组均未出现关节脱位,关节间隙正常。A组成骨细胞和成纤维细胞VEGF免疫反应性高,骨缺损区完全修复,大部分材料被新生骨小梁替代,缺损区骨密度接近于周围骨组织;B组VEGF免疫反应性较A组低,骨缺损区大部分修复,新生骨小梁替代部分材料,骨小梁较A组纤细稀疏,缺损区骨密度低于周围骨组织。C组骨缺损区修复,与周围骨组织融合,自体松质骨周围大量胶原纤维包裹并有新骨生成。[结论]应用具有促进血管生成作用的掺锶聚磷酸钙修复兔激素性股骨头坏死骨缺损过程中,可通过诱导VEGF表达促进新生血管生成加速骨修复过程。

关键词(KeyWords)： 血管内皮细胞生长因子;股骨头坏死;骨缺损;修复;掺锶聚磷酸钙

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 赵海燕;夏亚一;康鹏德;

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参考文献(References)：

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