戴闽;程明;刘虎诚;程细高;范红先;张斌;

• 1:南昌大学第一附属医院骨二科

摘要(Abstract)：

[目的]观察不同浓度金属磨损颗粒对RAW264.7在体外分化成破骨细胞的影响,明确浓度与破骨细胞分化数量的关系。[方法]真空球磨法制备人工关节磨损颗粒:RANKL诱导RAW264.7体外分化成破骨细胞,通过TRAP染色,电镜扫描检测骨片吸收陷窝来鉴定破骨细胞;不同浓度人工关节磨损颗粒混悬液作用RAW264.7,并用RANKL诱导后第7d,TRAP染色后,光镜下计数破骨细胞数量。[结果]不同浓度磨损颗粒作用于RAW264.77d后,显微镜下计数破骨细胞数量,结果显示随着磨损颗粒混悬液浓度增加,RANKL诱导生成的破骨细胞增多,低、中、高浓度3组破骨细胞数均显著高于空白对照组(P<0.05),中、高浓度组破骨细胞数均显著高于低浓组(P<0.05),高浓度组破骨细胞数亦显著高于中浓组(P<0.05)。[结论](1)RAW264.7是一种较好的破骨前体细胞模型,RAW264.7诱导形成破骨细胞的方法简便易行,所获得破骨细胞均一性好;(2)人工关节金属磨损颗粒为RAW264.7细胞向具有骨质吸收功能的破骨细胞转化发挥正向作用,而且与混悬液的浓度有量效关系。

关键词(KeyWords)： 人工关节,金属磨损颗粒;真空球磨,破骨细胞

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(编号:30760255)

作者(Author): 戴闽;程明;刘虎诚;程细高;范红先;张斌;

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