管大凡;陈月芹;刘洪美;张聪;李庆伟;陈国武;梁啸;孟纯阳;

• 1:新乡医学院
• 2:济宁医学院附属医院
• 3:济宁医学院病理教研室

摘要(Abstract)：

[目的]探讨携带h BMP2/h VEGF165双基因重组腺病毒共修饰BMSCs复合多孔n-HA/PA66修复兔桡骨大段骨缺损在不同时相点影像学动态变化和临床影像评估的可靠性。[方法]选取40只成年新西兰大白兔,雄性,体重2.8~3.1 kg,采用完全随机法分为2大组,每组20只,手术制作15 mm双侧桡骨中段骨缺损模型。A1组:左侧骨缺损处不植入任何材料为空白对照组;A2组:右侧骨缺损处植入h BMP2/BMSCs/n-HA/PA 66人工骨材料;B1组:左侧骨缺损处植入BMSCs/n-HA/PA66人工骨材料;B2组:右侧骨缺损处植入h BMP2/h VEGF165/BMSCs/n-HA/PA 66人工骨材料。分别于术后2、4、8、12周进行X线、CT三维重建观察和甲苯胺蓝组织染色观察。[结果]B2组各个时期Lane-Sandhu法X线射线评分均高于其他各组,差异有统计学意义(P<0.05),A2组优于B1组(P<0.05),A1组最差(P<0.05);且CT三维重建骨形成量和组织染色效果均优于同期其他各组。[结论]h BMP2/h VEGF165双基因重组腺病毒共修饰BMSCs复合多孔n-HA/PA66人工骨材料治疗兔大段骨缺损,影像学观察,直观、形象地显示了其持久、稳定、高效的骨修复作用,为基因治疗复合人工骨材料修复骨缺损提供了良好的实验依据。

关键词(KeyWords)： 骨形态发生蛋白2;血管内皮生长因子165;基因治疗;骨缺损修复;影像学研究

Abstract:

Keywords:

基金项目(Foundation): 山东省自然科学基金资助项目(编号:ZR2010HM090);; 山东省教育厅资助项目(编号:J14LL04)

作者(Author): 管大凡;陈月芹;刘洪美;张聪;李庆伟;陈国武;梁啸;孟纯阳;

Email:

DOI:

参考文献(References)：

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