恶性骨肿瘤来源成纤维母细胞促进溶骨作用Osteolysis effect by fibroblasts derived from malignant bone tumor
孙嗣国;马保安;周勇;张明华;范清宇;
摘要(Abstract)：
[目的]检验恶性骨肿瘤来源的成纤维母细胞是否能够支持破骨细胞分化及其溶骨作用。[方法]酶消化法从肿瘤组织中分离成纤维母细胞并作体外培养。细胞传代后,一部分成纤维母细胞与外周血单核细胞共培养,分别加入M-CSF、骨保护素(OPG)及抗TNFα中和抗体。同时,作者还应用了Transwell系统检验可溶性促溶骨性因子的表达。培养结束后检测抗酒石酸磷酸酶(TRAP)的表达及骨吸收陷窝的形成,并比较各组细胞的骨吸收活性。另一部分成纤维母细胞单独培养,RT-PCR法检测其细胞核因子кB受体活化因子配体(RANKL)和TNFα-mRNA的表达。[结果]在M-CSP存在的条件下,2种细胞共培养组分别于14、21 d观察到TRAP阳性的多核细胞和骨吸收陷窝形成;大剂量OPG彻底阻断TRAP阳性多核细胞及骨吸收陷窝的形成,而大剂量抗TNFα中和抗体对成纤维母细胞支持的破骨细胞形成未显示抑制作用。RT-PCR结果显示恶性骨肿瘤来源的成纤维母细胞能够表达RANKL和TNF-αmRNA。[结论]在M-CSF存在的条件下,恶性骨肿瘤来源的成纤维母细胞通过表达RANKL支持破骨细胞分化及其溶骨作用。
关键词(KeyWords)： 破骨细胞;溶骨作用;细胞核因子кB受体活化因子配体;成纤维母细胞
基金项目(Foundation):
作者(Author): 孙嗣国;马保安;周勇;张明华;范清宇;
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DOI:
参考文献(References)：
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