戴闽;周通华;熊皞;邹文楠;詹平;付文峰;

• 1:南昌大学第一附属医院骨二科
• 2:南昌大学建筑工程学院工程力学实验中心

摘要(Abstract)：

[目的]观察金属Co2+、Cr3+离子对小鼠成骨细胞(MC3T3E1)的表面超微结构、凋亡、细胞周期以及在Co2+、Cr3+离子刺激下对成骨细胞分泌ALP(碱性磷酸酶)的影响。[方法]体外培养成骨细胞。扫描电镜检测细胞形态学表面超微结构,Annexin V-AITC/PI双染色法检测细胞凋亡率,流式细胞术检测细胞周期分布。ELISA(酶联免疫分析)法对培养上清液ALP含量进行检测。[结果]Co2+Cr3+金属离子明显促进MC3T3凋亡,且在24 h凋亡率最高;同时G2M分布比例明显降低,细胞停滞在G0G1期的比率明显增多;小鼠ALP Elisa试剂盒实验的OD值明显下降;扫面电镜照相显示细胞形态学表面超微结构有明显差异。[结论]Co2+、Cr3+离子对成骨细胞的细胞周期分布有明显影响,抑制其增殖使细胞大部分处于休眠期(G0G1期),同时增加其凋亡率,并可抑制成骨细胞释放ALP。

关键词(KeyWords)： 金属离子;成骨细胞;细胞表面超微结构;细胞周分布;细胞凋亡率;碱性磷酸酶

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(编号:30760255)

作者(Author): 戴闽;周通华;熊皞;邹文楠;詹平;付文峰;

Email:

DOI:

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