魏帅;程晓清;王玉;王维山;丁晓;韩公海;刘平;彭江;史晨辉;

• 1:石河子大学医学院第一附属医院骨科中心
• 2:解放军总医院全军骨科研究所

摘要(Abstract)：

[目的]比较大鼠股神经分支基膜管结构和成分差异,为组织工程神经引导周围神经损伤后的精确再生提供依据。[方法]选取健康8周龄雄性SD大鼠30只,随机分为2组,实验组取股神经及其分支进行化学去细胞处理;对照组取新鲜股神经及其分支;观察指标为超微结构、组织学、免疫组织化学、拉曼光谱特征。[结果](1)超微结构:对照组股神经肌支髓鞘厚度大于皮支(1.28±0.41)μm vs (0.81±0.32)μm,(P<0.05),对照组股神经肌支基底膜厚度大于股神经皮支(34.55±2.23) nm vs (31.86±3.47) nm,(P<0.05);扫描电镜显示实验组去细胞处理后的取向性结构管状结构,无可见细胞结构;对照组扫描电镜显示可见明显分束的神经纤维及细胞结构;(2)组织学及免疫荧光显示:对照组股神经肌支乙酰胆碱酯酶染色阳性部位所占比例较大,皮支染色阴性部位所占比例较大;实验组去细胞处理后HE神经纤维呈均一红染管状细胞外基质结构,无蓝色可见细胞核结构;实验组神经去细胞处理后DAPI和Laminin染色为红色层粘连蛋白背景,无亮蓝色细胞核结构;(3)拉曼光谱分析显示:实验组神经去细胞处理后股神经肌支和皮支波峰出现位置无差异,主要为脂类和蛋白质及微量核酸。[结论]大鼠股神经肌支主要含运动神经纤维,皮支主要含感觉神经纤维;肌支和皮支的基膜管在结构上存在差异,其拉曼光谱特征基本相似。

关键词(KeyWords)： 股神经;股神经肌支;股神经皮支;基底膜;基膜管;拉曼光谱

Abstract:

Keywords:

基金项目(Foundation): 国家973项目(编号:2014CB542201);; 国家重点研发计划项目(编号:2017YFA0104702,2016YFC1101601)

作者(Author): 魏帅;程晓清;王玉;王维山;丁晓;韩公海;刘平;彭江;史晨辉;

Email:

DOI:

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