张楠;张柳;郑桓;赵小林;李硕;张胜强;刘志翔;

• 1:华北煤炭医学院附属医院骨科
• 2:华北煤炭医学院附属医院骨科
• 3:华北煤炭医学院附属医院骨科
• 4:华北煤炭医学院附属医院骨科

摘要(Abstract)：

[目的]研究降钙素(calcitonin,CT)对骨性关节炎关节软骨的保护作用。[方法]32只6个月龄新西兰大白兔,雌雄各半,行右膝关节前交叉韧带切断术。术后1周将动物随机分为实验组和对照组。实验组皮下注射鲑鱼降钙素5IU/(kg.d),连续6周;对照组则给予等剂量盐水。术后7周处死动物。取股骨内髁制成切片行HE、番红-固绿和MMP-3免疫组化染色。取膝关节软骨行胶原酶消化法提取软骨细胞进行体外培养,于第Ⅱ代融合90%后提取mRNA,采用实时荧光定量PCR方法检测MMP-3,aggrecan的表达。HE切片Mankin评分。番红-固绿和MMP-3免疫组化染色切片用图像分析仪测量平均灰度值。PCR结果记录Ct值。[结果]实验组关节软骨的组织学Mankin评分结果,番红-固绿和MMP-3免疫组化染色平均灰度值测量结果低于对照组(P<0.05);实验组体外培养软骨细胞的MMP-3的RNA含量低于对照组(P<0.05),aggrecan的RNA含量高于对照组(P<0.05)。[结论]降钙素5IU/(kg.d)皮下注射能够增加体外培养软骨细胞分泌的aggrecan的含量以及下调MMP-3的表达;可能通过体内明显减轻兔膝关节软骨基质的降解,减少MMP-3的表达而保护软骨。

关键词(KeyWords)： 降钙素;骨性关节炎;基质金属蛋白酶3;软骨细胞

Abstract:

Keywords:

基金项目(Foundation): 河北省科技领军人才创新基金项目(NO.06547008D-8)

作者(Author): 张楠;张柳;郑桓;赵小林;李硕;张胜强;刘志翔;

Email:

DOI:

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