SPIO标记骨髓间充质干细胞和软骨细胞共培养的研究Research of co-culture of chondrocyts and bone marrow mesenchymal stem cells labeled by SPIO in vitro
詹兴旺;姜艳;王文良;
摘要(Abstract):
[目的]探讨SPIO标记骨髓间充质干细胞和软骨细胞共培养的可行性,为临床修复关节软骨损伤寻找新途径。[方法]分离、扩增新西兰大白兔骨髓间充质干细胞(BMSCs)和软骨细胞,根据SPIO标记(50μg/m l)和不同培养环境(共培养、单独培养)分4组,每天在倒置显微镜观察共培养后BMSCs的形态变化,共培养14 d后,免疫组化检测Ⅱ型胶原表达情况,阿利新蓝法检测蛋白多糖(GAG)表达水平。[结果]共培养7 d后标记的部分BM-SCs变圆,14 d时BMSCs形态高度分化与成熟软骨细胞相似,其蛋白多糖和Ⅱ型胶原的基因转录和蛋白表达均增高,明显优于对照组,差异具有显著性意义(P<0.01)。[结论]1.软骨细胞微环境能有效诱导BMSCs向软骨细胞分化;2.SPIO可以安全、有效地标记BMSCs。
关键词(KeyWords): SPIO;骨髓间充质干细胞;软骨细胞;共培养
基金项目(Foundation): 国家自然科学基金项目(编号:30740089);; 天津市自然科学基金(编号:10JCYBJC10800);; 武警医学院科研基金项目(编号:WZK200902)
作者(Author): 詹兴旺;姜艳;王文良;
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DOI:
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