许海委;徐宝山;杨强;李秀兰;张杨;马信龙;夏群;张春秋;伍耀宏;

• 1:天津医科大学研究生院
• 2:天津市天津医院
• 3:天津理工大学

摘要(Abstract)：

[目的]分离培养兔脂肪基质干细胞(adipose-derived stem cells,ADSCs),观察在TGF-β1,bFGF,IGF联合诱导下其生物学特性及向软骨细胞定向分化的能力。[方法]取4个月龄新西兰大白兔颈背部脂肪组织,分离、培养ADSCs。选取第3代,分为两组,实验组用软骨诱导液(含TGF-β1、bFGF、IGF)对细胞进行培养,对照组用普通高糖培养液培养,倒置显微镜观察两组细胞形态,MTT测定其增殖情况;14 d后行组织学观察(包括甲苯胺蓝染色、Ⅱ型胶原免疫组化),并实时荧光定量PCR检测两组细胞Ⅱ型胶原、蛋白聚糖、SOX 9基因的表达情况。[结果]实验组细胞逐渐变为软骨样细胞形态,增殖速度明显加快,14 d后实验组甲苯胺蓝染色、Ⅱ型胶原免疫组化阳性,对照组为阴性,荧光定量PCR结果显示实验组Ⅱ型胶原、蛋白聚糖、SOX 9的基因转录水平明显高于对照组。[结论]在TGF-β1,bFGF,IGF联合诱导条件下ADSCs增殖迅速,可高表达Ⅱ型胶原、蛋白聚糖、SOX 9,向软骨细胞定向分化。

关键词(KeyWords)： 转化生长因子-β1;碱性成纤维细胞生长因子;胰岛素样生长因子;脂肪基质干细胞;软骨细胞

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(编号:31000432,11172208);; 天津市卫生局科技基金重点资助项目(编号:2010KR08)

作者(Author): 许海委;徐宝山;杨强;李秀兰;张杨;马信龙;夏群;张春秋;伍耀宏;

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参考文献(References)：

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