孙成良;Liu Chuanju;李栋;张伟;杨光;姜鹏;

• 1:山东大学附属省立医院骨科
• 2:New York University School of Medicine 301 East 17th Street New York
• 3:NY10003

摘要(Abstract)：

[目的]探讨人骨髓核分化抗原(MNDA)对人骨肉瘤Saos-2细胞增殖及侵袭的影响。[方法]逆转录聚合酶链反应(RT-PCR)法定量测定Saos-2细胞中MNDA的mRNA表达差异。将MNDA基因转染Saos-2细胞,MTT法测定检测细胞增殖程度的改变,Transwell小室法测定肿瘤细胞侵袭能力的改变。没有转染质粒的和转染空质粒的Saos-2做为对照组。[结果]MNDA的mRNA在骨肉瘤Saos-2细胞中表达明显降低。转染MNDA的人骨肉瘤Saos-2细胞的增殖率低于对照组,并且抑制能力随时间延长抑制而增加,侵袭穿透能力减弱。[结论]人骨肉瘤转染MNDA可抑制肿瘤细胞的增殖,降低肿瘤细胞的侵袭穿透能力。

关键词(KeyWords)： MNDA;骨肉瘤;Saos-2细胞;细胞增殖;细胞侵袭

Abstract:

Keywords:

基金项目(Foundation): 山东省自然科学基金(编号:ZR2009CM042)

作者(Author): 孙成良;Liu Chuanju;李栋;张伟;杨光;姜鹏;

Email:

DOI:

参考文献(References)：

• [1]Marulanda GA,Henderson ER,Johnson DA,et al.Orthopedic surgery options for the treatment of prim ary osteosarcoma[J].Cancer Control,2008,1:13-20.
• [2]Kim HJ,Chalmers PN,Morris CD.Pediatric osteogenic sarcoma[J].Curr Opin Pediatr,2010,1:61-66.
• [3]Briggs RC,Shults KE,Flye LA,et al.Dysregulated human myeloid nuclear differentiation antigen expression in myelodysplastic syndromes:evidence for a role in apoptosis[J].Cancer Res,2006,66:4645-4651.
• [4]Doggett KL,Briggs JA,Linton MF,et al.Retroviral mediated expression of the human myeloid nuclear antigen in a null cell line upregulates Dlk1 expression[J].J Cell Bioch,2002,86:56-66.
• [5]孙成良,Liu Chuanju,李栋,等.人骨髓核分化抗原对人骨肉瘤Saos-2细胞凋亡和增殖的影响[J].中国矫形外科杂志,2013,3:267-230.
• [6]Pola E,Gao W,Zhou Y,et al.Efficient bone formation by gene transfer of human LIM mineralization protein-3[J].Gene Ther,2004,8:683-693.
• [7]Hakansson PD,Segal C,Lassen U,et al.Meltzer:identification of genes differentially regulated by the P210 BCR/ABL1 fusion oncogene using cDNA microarrays[J].Exp Hematol,2004,32:476-482.
• [8]Todesco A,Carli M,Iacona I,et al.All-trans retinoic acid and interferon alpha in the treatment of a patient w ith resistant metastatic osteosarcoma[J].Cancer,2000,12:2661-2666.
• [9]Strander H,Bauer HC,Brosjo O,et al.Long term dajuvant interferon treatment of human osteosarcoma.A polot study[J].Acta Oncol,1995,6:877.
• [10]Deschamps S,Meyer J,Chatterjee G,et al.The mouse Ifi200 gene cluster:genomic sequence,analysis,and comparison with the human HIN-200 gene cluster[J].Genomics,2003,1:34-46.
• [11]Ludlow LE,Johnstone RW,Clarke CJ.The HIN-200 family:more than interferon-inducible genes[J].Exp Cell Res,2005,1:1-17.
• [12]Alimirah F,Chen J,Davis FJ,et al.IFI16 in human prostate cancer[J].Mol Cancer Res,2007,3:251-259.
• [13]Song LL,Alimirah F,Panchanathan R,et al.Expression of an IFNinducible cellular senescence gene,IFI16,is up-regulated by p53[J].Mol Cancer Res,2008,11:1732-1741.
• [14]De Andrea M,Gioia D,Mondini M,et al.Effects of IFI16 overexpression on the growth and doxorubicin sensitivity of head and neck squamous cell carcinoma-derived cell lines[J].Head Neck,2007,9:835-844.
• [15]Briggs RC,Briggs JA,Ozer J,et al.The human myeloid cell nuelear differentiation antigen gene is one of at least two related interferoninducible genes located on chromosome lq that are expressed specifically in hematopoietic cells[J].Blood,1994,83:2153-2162.
• [16]郭文,卢翔,施鑫,等.α2整合素抗体对人类骨肉瘤MG63细胞迁移侵袭的影响[J].医学研究生学报,2009,12:1252-1255.