骨关节炎软骨及软骨下骨吡啶并林含量的变化Pyridinoline Concentration Changes in Osteoarthritic Articular Cartilage and Subchondral Bone-An Experimental Study
陆宁,曾维权,潘瑾,卢世璧
摘要(Abstract)：
目的 :测定骨关节炎 (OA)软骨及软骨下骨中吡啶并林 (Pyr)含量的变化 ,判断Pyr在OA诊断和疾病活跃程度评价方面的作用。方法 :复制成年兔膝关节OA模型 ,取胫骨内髁主要负重区关节软骨及软骨下骨 ,采用高效液相色谱法 (HPLC)测定其Pyr含量。结果 :8周内 ,关节软骨中Pyr含量逐渐下降 ;软骨下骨中Pyr含量在模型术后早期下降 ,随后逐渐升高。结论 :OA关节软骨及软骨下骨的Pyr含量随病程发生变化 ,可能是造成经尿液排Pyr升高的原因。尿液中Pyr含量的测定可望用于OA诊断及评价疾病的活跃程度。
关键词(KeyWords)： 吡啶并林;骨关节炎;关节软骨;软骨下骨
基金项目(Foundation):
作者(Author): 陆宁,曾维权,潘瑾,卢世璧
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参考文献(References)：
- 〔1〕 MacDonaldAG ,McHenryP ,RobinsSP ,ReidDM :Rela tionshipofurinarypyridiniumcrosslinkstodiseaseextentandactivityinosteoarthritis[J] .BrJRheumatol ,1994,3 3 :16～ 9.
- 〔2〕 ThompsonPW ,SpectorTD ,JamesIT ,etal.Urinarycol lagencrosslinksreflecttheradiographicseverityofkneeos teoarthritis[J] .BrJRheumatol,1992 ,3 1:75 9～ 61.
- 〔3〕 AstburyC ,BirdHA ,MclarenAM ,RobinsSP .Urinaryexcertionofpyridiniumcrosslinksofcollagencorrelatedwithjointdamageinarthritis[J] .BrJRheumatol ,1994,3 3 :11～ 5 .
- 〔4〕 HulthA ,LindbergL ,TelhogH .Experimentalosteoarthritisinrabbit .preliminaryreport .ActaOrthoScand[J] .1970 ,41:5 2 2～ 3 0 .
- 〔5〕 吴海山 ,钱其荣 ,顾其胜 .关节内注射透明质酸钠预防免关节骨关节炎的实验研究 [J] .中华骨科杂志 ,1996,16( 1) :3 7～ 9.
- 〔6〕 BlackD ,DuncanA ,RobinsSP .Quantitativeanalysisofthepyridiniumcrosslinksofcollageninurineusingion -pairedreversed phasehigh preformanceliquidchromatography[J] .AnalBiochem .1988,169:197～ 2 0 3 .
- 〔7〕 TakahashiM ,KushidaK ,HoshinoH ,etal .Concentra tionof pyridinolineanddeoxypyridinolineinjointtissuesfrompatientswithosteoarthritisorrheumatoidarthritis[J] .AnnRheumDis ,1996,5 5 :3 2 4～ 7.
- 〔8〕 ClaessensAAMC ,SchoutenJSAG ,VandenOuwelandFA ,ValkenburyHA .Doclinicalfindingsassociatewithradio graphicosteoarthritisoftheknee ?AnnRheumDis[J] .1990 ,49:771～ 4.
- 〔9〕 SpectorTD ,HartDT ,HuskissonEC .Theuseofradiog raphsinassessingtheseverityofkneeosteoarthritis[J] .JRheumatolsuppl,1991,18:3 8～ 9.
- 〔10〕EyreDR ,WuJ -J ,WoodsPE .Thecartilagecollagens :structuralandmetabolicstudies[J] .JRheumatolsuppl,1991,18:49～ 5 1.
- 〔11〕SeibelMJ ,DuncanA ,RobinsSP .Urinaryhydroxypyridi niumcrosslinksprovideindicesofcartilageandboneinvol vementinarthriticdiseases[J] .JRheumatol,1989,16:964～ 70 .
- 〔12〕BeardsworthLJ ,EyreDR ,DickinsonIR .Changeswithageintheurinaryexcretionoflysyl andhydroxyly sylpyridinoline ,twonewsmarkersofbonecollagenturnover[J] .JBoneMineralRes ,1990 ,15 :671.
- 〔13〕MclarenAm ,IsdaleAH ,WhitingPH ,etal.Physiologicalvariationintheerinaryexcretionofpyridiniumcrosslinksofcollagen .BrJRheumatol[J] .1993 ,3 2 :3 0 7～ 12 .
- 〔14〕SinigagliaL ,VarennaM ,BinelliL ,etal .Urinaryandsynovial pyridiniumcrosslinkconcentrationsin patientswithrheumatoidarthritisandosteoarthritis [J ] .AnnRheumDis ,1995 ,5 4:14 4～ 7.
- 〔15〕SinigagliaL ,VarennaM ,BinelliL .Tissuecrosslinkscon centrationinnormaljointsandchronicarticulardiseases[J] .AnnRheuDis ,1997,5 6:3 3 7～ 8.
- 〔16〕TakahashiM ,KushidaK ,OhishiT ,etal.Quantitativeanalysisofcrosslinkspyridinolineandpentosidineinarticu larcartilageofpatientswithboneandjointdisorders[J] .ArthritisRheum ,1994,5 :72 4～ 8.