刘洋;朱书涛;李国军;王晓;张永乐;

• 1:河南大学淮河医院骨科

摘要(Abstract)：

[目的]利用微重力培养环境,构建高质量组织工程化椎间盘。[方法]选用成年新西兰大白兔椎间盘细胞作为种子细胞,复合聚乳酸-羟基乙酸(PLGA)支架,分别在微重力培养环境和普通培养板环境下培养,利用倒置显微镜、MTT比色法、扫描电镜、组织学观察椎间盘细胞、支架和复合结构质量。[结果]体外单层培养的椎间盘细胞呈多角形符合传代要求,微重力环境比普通培养环境能更好地促进椎间盘细胞的增殖,同时椎间盘细胞在支架内分布更加均匀。[结论]微重力培养环境更适于椎间盘细胞在三维培养结构中均匀增殖,有利于构建高质量组织工程化椎间盘用于深入研究。

关键词(KeyWords)： 微重力;细胞培养;椎间盘;组织工程;髓核细胞

Abstract:

Keywords:

基金项目(Foundation): 河南省科技厅重点攻关项目(编号:112102310286)

作者(Author): 刘洋;朱书涛;李国军;王晓;张永乐;

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DOI:

参考文献(References)：

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