于德水;吕刚;曹阳;王岩峰;智晓东;范仲凯;

• 1:辽宁医学院附属第一医院骨科
• 2:中国医科大学附属第一医院骨科

摘要(Abstract)：

[目的]研究骨髓基质细胞(BMSCs)移植对大鼠脊髓损伤(SCI)后血管内皮生长因子(VEGF)蛋白表达及神经细胞凋亡的影响,探讨骨髓基质细胞移植治疗脊髓损伤的机制。[方法]取大鼠股骨和胫骨骨髓培养BM-SCs并传代。参照Taoka′s方法制作大鼠脊髓压迫损伤模型。脊髓损伤后30 min,进行BMSCs或DMEM培养液损伤周围注射。术后3、7和14 d,应用Western Blot法检测损伤脊髓组织内VEGF蛋白的表达,应用TUNEL方法检测脊髓损伤周围神经细胞凋亡情况。[结果]移植术后3、7、14 d,BMSCs组VEGF蛋白的表达水平呈时间依赖性增加,与DMEM组相比,术后3 d两组VEGF蛋白表达水平没有显著性差异(P>0.05),而术后7和14 d,BMSCs组VEGF蛋白表达水平显著高于DMEM组(P<0.01)。此外,与DMEM组大鼠相比,移植术后3、7和14 d,BMSCs移植显著减少脊髓损伤周围区凋亡的神经细胞数目(P<0.05)。[结论]脊髓损伤后,BMSCs移植能够增加VEGF蛋白的表达并且抑制神经细胞凋亡。

关键词(KeyWords)： 脊髓损伤;骨髓基质细胞;细胞移植;血管内皮生长因子;凋亡

Abstract:

Keywords:

基金项目(Foundation): 教育部高校博士学科点专项科研基金项目(编号:20060159019);; 辽宁医学院第一临床学院青年科技启动基金(2010)

作者(Author): 于德水;吕刚;曹阳;王岩峰;智晓东;范仲凯;

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