程明;戴闽;祝荫;程细高;范红先;刘虎诚;

• 1:南昌大学第一附属医院骨二科
• 2:南昌大学第一附属医院消化科

摘要(Abstract)：

[目的]建立人骨髓来源的间充质干细胞(hBMSCs)分离、培养及传代的方法,观察成hBMSCs体外成骨潜能。[方法]采用全骨髓贴壁筛选法分离培养hBMSCs,流式细胞仪检测细胞表型;所得细胞第3代用含100 nmol/L地塞米松、5mmol/Lβ-甘油磷酸钠,50μg/ml抗坏血酸的条件培养基进行骨诱导,茜素红染色鉴定。[结果]分离培养的细胞流式细胞术检测显示CD44、CD105阳性,而CD34、CD45阴性,符合MSCs特征;hBMSCs经骨诱导后可形成钙结节,茜素红染色显示阳性。[结论]全骨髓贴壁筛选法可分离获得高纯度的hBMSCs,其在体外具有成骨潜能。

关键词(KeyWords)： 间充质干细胞;骨诱导;成骨细胞

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(编号:81060198)

作者(Author): 程明;戴闽;祝荫;程细高;范红先;刘虎诚;

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参考文献(References)：

• [1]Pittenger MF,Mackay AM,Beck SC,et al.Multilineage potential ofadult human mesenehymal stem cells[J].Science,1999,5411:143-147.
• [2]Pittenger MF.Mesenchymal stem cells from adult bone marrow[J].Methods Mol Biol,2008,449:27-44.
• [3]Chamberlain G,Fox J,Ashton B,et al.Concise review:mesenchymalstem cells:their phenotype,differentiation capacity,immunologicalfeatures,and potential for homing[J].Stem Cells,2007,25:2739-2749.
• [4]Caballero M,Reed CR,Madan G,et al.Osteoinduction in umbili-cal cord-and palate periosteum-derived mesenchymal stem cells[J].Ann Plast Surg,2010,5:605-609.
• [5]Markway BD,Tan GK,Brooke G,et al.Enhanced chondrogenicdifferentiation of human bone marrow-derived mesenchymal stemcells in low oxygen environment micropellet cultures[J].Cell Trans-plant,2010,1:29-42.
• [6]Vashi AV,Keramidaris E,Abberton KM,et al.Adipose differentia-tion of bone marrow-derived mesenchymal stem cells using pluron-ic F-127 hydrogel in vitro[J].Biomaterials,2008,5:573-579.
• [7]Psaltis PJ,Zannettino AC,Worthley SG,et al.Concise review:mesenchymal stromal cells:potential for cardiovascular repair[J].Stem Cells,2008,9:2201-2210.
• [8]Li JM,Zhu H,Lu S,et al.Migration and differentiation of humanmesenchymal stem cells in the normal rat brain[J].Neurol Res,2010:19.