骨髓间质干细胞与Ⅱ型胶原修饰的PLGA黏附性的观察Adhesion of bone marrow mesenchymal stem cells cultured on PLGA modified with Ⅱ collagen
张利;查振刚;周长忍;姚平;吴昊;林宏生;杨淑野;王双利;
摘要(Abstract)：
[目的]优化骨髓间质干细胞(bone marrow mesenchymal stem cells,BMSCs)体外分离培养技术,改良生物材料聚乙醇酸-乳酸共聚物(poly lactide-co glycolide,PLGA)的细胞黏附性,观察BMSCs与Ⅱ型胶原修饰的PL-GA的黏附性。[方法]密度梯度离心法分离BMSCs,流式细胞分析法(FACS)对细胞表面抗原、细胞活力、细胞周期进行检测,相差显微镜观察细胞形态。相分离法制做PLGA,Ⅱ型胶原进行表面修饰,取第三代干细胞与PLGA附和,扫描电镜观察细胞与材料的黏附情况。[结果]BMSCs可在体外分离扩增,表达CD29、CD44和CD106,不表达CD34和CD45,细胞活力为88.96%,G0-G1细胞占90.32%。细胞形态为长梭形,Ⅱ型胶原表面修饰的PLGA平均孔径为100μm,与BMSCs有较好的黏附性。[结论]BMSCs可在体外长期、稳定培养,是理想的组织工程种子细胞。Ⅱ型胶原表面修饰的PLGA与干细胞有较好的黏附性,可用来做组织工程生物材料。
关键词(KeyWords)： 骨髓间质干细胞;PLGA;组织工程
基金项目(Foundation): 广东省科技攻关项目(2006B60501009);; 广州市科技资助项目(2006Z3-E5211)
作者(Author): 张利;查振刚;周长忍;姚平;吴昊;林宏生;杨淑野;王双利;
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