高旭鹏;郭志远;孙逊;王玉;彭江;郭全义;赵庆;卢世璧;

• 1:解放军总医院骨科研究所

摘要(Abstract)：

[目的]探讨经尾静脉移植骨髓间充质干细胞对SD大鼠周围神经Crush损伤的修复作用。[方法]建立SD大鼠5 mm坐骨神经Crush损伤模型,然后将实验动物分为两组:干细胞治疗组和对照组(生理盐水组)。Crush损伤模型建立后1周,经尾静脉分别注射5×106干细胞(干细胞治疗组)或生理盐水(生理盐水组),然后于不同的时间点分别借助于活体荧光成像系统、坐骨神经指数测量、电生理检测和神经肌肉病理学等方法评价干细胞的归巢现象及其对神经Crush损伤的修复作用。[结果]活体荧光成像系统检测到干细胞治疗组的损伤侧神经Wallerian变性区域有高强度荧光信号聚集;SFI测量证实在损伤后2、4、6周三个不同时间点,干细胞治疗组SFI的恢复优于对照组;同样的,电生理检测和神经肌肉病理学均提示干细胞治疗组的促神经损伤恢复效果优于对照组。[结论]经尾静脉移植骨髓间充质干细胞可以促进周围神经Crush损伤的修复。

关键词(KeyWords)： 静脉移植;骨髓间充质干细胞;神经损伤;Crush损伤;Wallerian变性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(编号:31170946);; 国家863主题项目(编号:2012AA020502);; 全军十二五重点项目(编号:BWS11J025)

作者(Author): 高旭鹏;郭志远;孙逊;王玉;彭江;郭全义;赵庆;卢世璧;

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参考文献(References)：

• [1]蒋华军,巍曲.周围神经缺损修复研究进展[J].中国矫形外科杂志,2009,22:1707-1709.
• [2]许文静,于海龙,孙明学,等.化学去细胞同种异体神经移植术后病人的护理[J].中国矫形外科杂志,2008,5:379-380.
• [3]Mahmood A,Lu D,Lu M,et al.Treatment of traumatic brain injury in adult rats with intravenous administration of human bone marrow stromal cells[J].Neurosurgery,2003,3:697-702.
• [4]Seyfried D,Ding J,Han Y,et al.Effects of intravenous administration of human bone marrow stromal cells after intracerebral hemorrhage in rats[J].J Neurosurg,2006,2:313-318.
• [5]Zhang J,Li Y,Lu M,et al.Bone marrow stromal cells reduce axonal loss in experimental autoimmune encephalomyelitis mice[J].J Neurosurg Res,2006,3:587-595.
• [6]Zhao Z,Wang Y,Peng J,et al.Repair of nerve defect with acellular nerve graft supplemented by bone marrow stromal cells in mice[J].Microsurgery,2011,5:388-394.
• [7]Marconi S,Castiglione G,Turano E,et al.Human adipose-derived mesenchymal stem cells systemically injected promote peripheral nerve regeneration in the mouse model of sciatic crush[J].Tissue Eng,2012,11-12:1264-1272.
• [8]Wang W,Jiang Q,Zhang H,et al.Intravenous administration of bone marrow mesenchymal stromal cells is safe for the lung in a chronic myocardial infarction model[J].Regen Med,2011,2:179-190.
• [9]Li Y,Yao Y,Sheng Z,et al.Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction[J].Int Nanomed,2011,6:815-823.
• [10]Constantin G,Marconi S,Rossi B,et al.Adipose-derived mesenchymal stem cells ameliorate chronic experimental autoimmune encephalomyelitis[J].Stem Cells,2009,10:2624-2635.
• [11]Wang Y,Deng Y,Zhou GQ.SDF-1alpha/CXCR4-mediated migration of systemically transplanted bone marrow stromal cells towards ischemic brain lesion in a rat model[J].Brain Res,2008,21:104-112.
• [12]Lu DY,Yi L,Wang L,et al.Intraarterial administration of marrow stromal cells in a rat model of traumatic brain injury[J].J Neurotrauma,2001,8:813-819.
• [13]Chen JL,Yi L,Wang L,et al.Therapeutic benefit of intracerebral transplantation of bone marrow stromal cells after cerebral ischemia in rats[J].J Neurol Sci,2001,189:49-57.
• [14]Ji JF,He BP,Dheen ST,et al.Interactions of chemokines and chemokine receptors mediate the migration of mesenchymal stem cells to the impaired site in the brain after hypoglossal nerve injury[J].Stem Cells,2004,22:415-427.
• [15]Son BR,Marquez-Curtis L,Kucia M,et al.Migration of bone marrow and cord blood mesenchymal stem cells in vitro is regulated by stromal-derived factor-1-CXCR4 and hepatocyte growth factorc-met axes and involves matrix metalloproteinases[J].Stem Cells,2006,24:1254-1264.
• [16]Zarbakhsh S,Bakhtiyari M,Faghihi A,et al.The effects of schwann and bone marrow stromal stem cells on sciatic nerve injury in rat:a comparison of functional recovery[J].Cell J(Yakhteh),2012,1:39-46.
• [17]Ni HC,Tseng TC,Chen JR,et al.Fabrication of bioactive conduits containing the fibroblast growth factor 1 and neural stem cells for peripheral nerve regeneration across a 15 mm critical gap[J].Biofabrication,2013,3:035010.
• [18]Pluchino S,Zanotti L,Rossi B,et al.Neurosphere-derived multipotent precursors promote neuroprotection by an immunomodulatory mechanism[J].Nature,2005,7048:266-271.