王波;姜伟;刘海鹰;陈定宝;郑姝颖;

• 1:北京大学人民医院脊柱外科
• 2:山东省郓城县人民医院骨科
• 3:北京大学人民医院脊柱外科
• 4:北京大学人民医院病理科

摘要(Abstract)：

[目的]研究重组人红细胞生成素(recombinant human erythropoietin,rh-EPO)对脊髓冲击性损伤的治疗效果。[方法]24只新西兰白兔采用重物坠落的方法造成脊髓冲击性损伤。损伤12 h后,对照组静脉给予生理盐水;小剂量组、中等剂量组和大剂量组分别静脉给予rh-EPO 100、500、1000 IU/kg。损伤后24 h、48 h、1周评估下肢神经功能。损伤后1周时处死动物,脊髓标本进行HE和Caspase-3组化染色,电子显微镜评估超微结构损伤。[结果]EPO治疗组的神经功能评分明显高于对照组,HE染色和电子显微镜显示组织和超微结构损伤明显轻于对照组,Caspase-3阳性细胞数明显少于对照组。中等剂量组和大剂量组的治疗效果无显著性差异。[结论]脊髓冲击性损伤后12 h给予人红细胞生成素可减轻脊髓的组织和超微结构继发性损伤,并可对抗神经细胞凋亡,促进脊髓功能恢复。中等剂量EPO是治疗脊髓损伤的适当选择。

关键词(KeyWords)： 重组人红细胞生成素;脊髓损伤;凋亡;超微结构

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 王波;姜伟;刘海鹰;陈定宝;郑姝颖;

Email:

DOI:

参考文献(References)：

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