噻莱昔布(celecoxib)对人骨肉瘤细胞MG-63增殖与凋亡的影响Effect of celecoxib on anti-proliferation and Inducing apoptosis of human osteosarcoma cell MG-63 line
冯尔宥,夏仁云,张怡元,盛健,林焱斌
摘要(Abstract)：
目的:研究环氧化酶2(cyclooxygenase2,COX2)抑制剂celecoxib对人骨肉瘤细胞MG63抑制增殖及诱导凋亡的作用。方法:MTT比色观察celecoxib的细胞毒性作用及其浓度依赖性;Hoechst33258荧光染色、透射电子显微镜和TUNEL观察细胞凋亡的形态学变化;流式细胞仪检测细胞凋亡率及其时间依赖性。结果:celecoxib分别以10、20、40、80μmol/L作用后,细胞生长受到不同程度抑制;荧光显微镜、电镜和TUNEL观察到细胞胞浆浓缩、核凝聚、核碎裂和凋亡小体形成。流式细胞仪显示celecoxib40μmol/L作用24、48、72h后,细胞凋亡率分别与对照组比较有非常显著性差异(P<0.01)。结论:celecoxib能抑制人MG63细胞增殖,诱导细胞凋亡。
关键词(KeyWords)： 环氧化酶2;骨肉瘤MG63细胞;凋亡
基金项目(Foundation):
作者(Author): 冯尔宥,夏仁云,张怡元,盛健,林焱斌
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参考文献(References)：
- 〔1〕EdwardGiovannucci,KathleenM,Egan,etal.AspirinandtheRiskof ColorectalCanceronWomen[J].N.Engl.J.Med,1995,333(10):609～614.
- 〔2〕HarrisRE,NamboodiriKK,FarrarWB.Nonsteroidalanti inflammatory drugsandbreastcancer[J].Epidemiology,1996,7(2):203～205.
- 〔3〕KerrJER,WyllieAH,CurrieAH,etal.Apoptosis,abasicbiological phenomenonwithwiderimplicationsintissuekinetics[J].BrJCanc er,1972,26(4):239～257.
- 〔4〕RobertW,McmurrayMD,KennethJ,etal.COX2inhibitors:today andtomorrow[J].AmJMedSci,2002,323(4):181～189.
- 〔5〕KawamoriT,RaoCV,SeibertK,etal.Chemopreventiveactivityof celecoxib,aspecialcyclooxygenase2inhibitor,againstcoloncarcino genesis[J].CancerRes,1998,58(3):409～412.
- 〔6〕AoLinHsu,TsuiTingChing,DaShengWang,etal.TheCyclooxygen ase2InhibitorCelecoxibInduceApoptosisbyBlockingAktActivation inHumanProstateCancerCellsIndependentlyofBcl2[J].JBiol Chem,2000,275(15):11397～11403.
- 〔7〕KardoshiA,BlumenthalIII,WangWJ,etal.Differentialeffectsofse lectiveCOX2inhibitorsoncellcycleregulationandproliferationof glioblastomacelllines[J].CancerBiolTher,2004,3(1):55～62.