骨水泥颗粒促进肿瘤坏死因子α诱导的破骨细胞形成及其骨吸收作用Effect of polymethy imethacry late particles on tumor necrosis factor α-induced osteoclast formation and bone resorption
孙嗣国,范清宇,周勇,马保安
摘要(Abstract)：
目的:验证骨水泥颗粒对肿瘤坏死因子α(TNFα)诱导的破骨细胞形成及其生物学活性的影响。方法:体外培养外周血单核细胞,对照组加入TNFα和白细胞介素-1α(IL-1α)及巨噬细胞克隆集落刺激因子(M-CSF),实验组并分别加入含有或不含有硫酸钙的骨水泥(PMMA±BaSO4)颗粒。对培养终末细胞作组织化学染色检测破骨细胞标志物抗酒石酸酸性磷酸酶(TRAP)的表达,并以象牙磨片上虫蚀样骨吸收陷窝的形成为指标检测破骨细胞的生物学活性;并比较各实验组中TRAP阳性多核细胞(multinucleatedcells,MNCs)及虫蚀样骨吸收陷窝形成时间的早晚。结果:各组TRAP阳性MNCs的数量无明显差异;PMMA±BaSO4组象牙磨片上骨吸收陷窝的面积均较对照组大,差异具有显著性(P<0.01);并且PMMA±BaSO4组TRAP阳性的MNCs及虫蚀样骨吸收陷窝的形成均较对照组早。结论:PMMA±BaSO4颗粒能够促进TNFα诱导的破骨细胞分化提早发生并促进其骨吸收活性。
关键词(KeyWords)： 骨水泥颗粒;肿瘤坏死因子α;破骨细胞;骨吸收作用
基金项目(Foundation):
作者(Author): 孙嗣国,范清宇,周勇,马保安
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