于召龙;王云浩;黄凯;晏鹏;田纪伟;

• 1:上海交通大学附属第一人民医院

摘要(Abstract)：

椎间盘位于椎体之间,由髓核、纤维环和软骨终板构成,其主要成分为蛋白多糖、胶原纤维、弹性蛋白和水。因其特殊的解剖结构,血液供应和营养状况有限以及常处于应力状态下,在外伤、职业、妊娠、遗传及先天发育异常等因素作用下,极易发生退变。随着椎间盘退变机制研究的深入,发现长链非编码RNA(LncRNA)可能通过调控髓核细胞凋亡、细胞外基质降解,参与炎症反应等过程,在椎间盘退变的发生、发展中起到重要作用。本文拟对长链非编码RNA的特点及其介导椎间盘退变的不同机制作一综述。

关键词(KeyWords)： 长链非编码RNA;椎间盘退变;凋亡;细胞外基质;炎症反应

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 于召龙;王云浩;黄凯;晏鹏;田纪伟;

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参考文献(References)：

• [1] Lan PH, Liu ZH, Pei YJ, et al. Landscape of RNAs in human lumbar disc degeneration[J]. Oncotarget, 2016, 7(39):63166-63176.
• [2] Chen WK, Yu XH, Yang W, et al. lncRNAs:novel players in intervertebral disc degeneration and osteoarthritis[J]. Cell proliferation, 2017, 50(1):1-10.
• [3] Munir S, Rade M, M??tt?JH, et al. Intervertebral disc biology:genetic basis of disc degeneration[J]. Current Molecular Biology Reports, 2018, 20(9):1-8.
• [4] Guttman M, Amit I, Garber M, et al. Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals[J]. Nature, 2009, 458(7235):223-227.
• [5] Guttman M, Rinn JL. Modular regulatory principles of large noncoding RNAs[J]. Nature, 2012, 482(7385):339-346.
• [6] Ponting CP, Oliver PL, Reik W. Evolution and functions of long noncoding RNAs[J]. Cell, 2009, 136(4):629-641.
• [7] Gibb EA, Brown CJ, Wan LL. The functional role of long non-coding RNA in human carcinomas[J]. Molecular Cancer, 2011, 10(1):38.
• [8] Ma L, Bajic VB, Zhang Z. On the classification of long non-coding RNAs[J]. Rna Biology, 2013, 10(6):924-933.
• [9] Wang K, Chang H. Molecular Mechanisms of Long Noncoding RNAs[J]. Molecular Cell, 2011, 43(6):904-914.
• [10] Derrien T, Johnson R, Bussotti G, et al. The GENCODE v7 catalog of human long noncoding RNAs:analysis of their gene structure,evolution, and expression[J]. 2012, 1(6):500-506.
• [11] Mercer TR, Mattick JS. Structure and function of long noncoding RNAs in epigenetic regulation[J]. Nat Structural Molecular Biol,2013, 20(3):300-307.
• [12] Maass PG, Rump A, Schulz H, et al. A misplaced lncRNA causes brachydactyly in humans[J]. J Clin Invest, 2012, 122(11):3990-4002.
• [13] Wan ZY, Song F, Sun Z, et al. Aberrantly expressed long noncoding RNAs in human intervertebral disc degeneration:a microarray related study[J]. Arthritis Res Ther, 2014, 16(5):465.
• [14] Qu Z, Quan Z, Zhang Q, et al. Comprehensive evaluation of differential lncRNA and gene expression in patients with intervertebral disc degeneration[J]. Molecular Medicine Reports, 2018, 10(9):1-8.
• [15] Zhang L, Niu T, Yang SY, et al. The occurrence and regional distribution of DR4 on herniated disc cells:a potential apoptosis pathway in lumbar intervertebral disc[J]. Spine, 2008, 33(4):422.
• [16]王毅峰. LncRNA在椎间盘退变过程中调控髓核细胞凋亡的功能及其机制研究[D].第二军医大学, 2016.
• [17] Chu K, Niu XH, Williams LT. A fas-associated protein factor,FAF1, potentiates fas-mediated apoptosis[J]. Proc Nat Acad Sci Am, 1995, 92(25):11894-11898.
• [18] Shiekhattar R. The Yin and Yang of enhancer like RNAs[J]. Embo J, 2013, 32(19):2533.
• [19] Ruan Z, Ma H, Li J, et al. The long non-coding RNA NEAT1 contributes to extracellular matrix degradation in degenerative human nucleus pulposus cells[J]. Exper Biol Med, 2018, 243(7):1535370218760774.
• [20] Yu C, Ni H, Zhao Y, et al. Potential role of lncRNAs in contributing to pathogenesis of intervertebral disc degeneration based on microarray data[J]. Med J Exper Clin Res, 2015, 21:3449.
• [21] Lee JH, Lee MJ. Emerging roles of the ubiquitin-proteasome system in the steroid receptor signalingv[J]. Arch Pharm Res, 2012,35(3):397-407.
• [22] Song J, Ahn C, Chun CH, et al. A long non-coding RNA, GAS5,plays a critical role in the regulation of miR-21 during osteoarthritisv[J]. J Orthop Res, 2014, 32(12):1628-1635.
• [23] Han Z, Wang J, Gao L, et al. Aberrantly expressed messenger RNAs and long noncoding RNAs in degenerative nucleus pulposus cells co-cultured with adipose-derived mesenchymal stem cellsv[J]. Arthritis Res Ther, 2018, 20(1):182.
• [24] Zhang C, Peng W, Jiang P, et al. Upregulation of lncRNA HOTAIR contributes to IL-1β-induced MMP overexpression and chondrocytes apoptosis in temporomandibular joint osteoarthritis[J].Gene, 2016, 586(2):248-253.
• [25] Kim D, Song J, Han J, et al. Two non-coding RNAs, MicroRNA-101 and HOTTIP contribute cartilage integrity by epigenetic and homeotic regulation of integrin-α1[J]. Cellular Signalling, 2013,25(12):2878-2887.
• [26] Cui H, Xie N, Tan Z, et al. The human long noncoding RNA lncIL7R regulates the inflammatory response[J]. Eur J Immunol,2014, 44(7):2085-2095.
• [27] Li Z, Chao T, Chang KY, et al. The long noncoding RNA THRIL regulates TNFαexpression through its interaction with hnRNPL[J]. Proc Nat Acad Sci, 2014, 111(3):1002-1007.
• [28] Li F, Sun J, Huang S, et al. LncRNA GAS5 Overexpression Reverses LPS-Induced Inflammatory Injury and Apoptosis Through UpRegulating KLF2 Expression in ATDC5 Chondrocytes[J]. Cellular Physiol Biochem, 2018, 45(3):1241-1251.
• [29] Krock E, Millecamps M, Ouellet J, et al.(112)Toll-like receptors in intervertebral disc degeneration and Pain:from man to mouse[J]. J Pain, 2017, 1:1-10.
• [30] Wang X, Lv G, Li J, et al. LncRNA-RP11-296A18.3/miR-138/HIF1A pathway regulates the proliferation ECM synthesis of human nucleus pulposus cells(HNPCs)(?)[J]. J Cellular Biochem, 2017, 118(12):201-207.
• [31] Chen J, Jia YS, Liu GZ, et al. Role of LncRNA TUG1 in intervertebral disc degeneration and nucleus pulposus cells via regulating Wnt/β-catenin signaling pathway[J]. Biochem Biophysical Res Commun, 2017, 25(1):72-77.