李斌;张伟;王健;孙水;吴帅;

• 1:山东大学附属省立医院关节外科

摘要(Abstract)：

[目的]探讨兔骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)体外诱导形成软骨细胞的方法,以及转化生长因子-β1(transforming growth factorβ1,TGF-β1)、胰岛素样生长因子-Ⅰ(insulin-like growth factor-Ⅰ,IGF-Ⅰ)和碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)之间的相互作用。[方法]对兔骨髓间充质干细胞体外进行原代和传代培养,按加入诱导条件不同分为四组:A组:TGF-β1+bFGF;B组:TGF-β1+IGF-Ⅰ;C组:TGF-β1;D组:空白对照组。3周后分别做四唑盐(methyl thiazolyl tetrazolium,MTT)比色试验、糖胺聚糖(glycosaminoglycan,GAG)检测和免疫组织化学染色。[结果]A、B、C三组Ⅱ型胶原免疫组织化学检测阳性;MTT吸光度值和GAG含量检测结果均为:A组>C组>D组,B组>C组>D组,统计学有显著差异。[结论]兔BMSCs在一定条件下可以诱导成软骨细胞,TGF-β1和IGF-Ⅰ、bFGF在BMSCs向软骨细胞分化和增殖时起协同作用。

关键词(KeyWords)： 骨髓间充质干细胞;软骨细胞;转化生长因子-β1;胰岛素样生长因子-Ⅰ;碱性成纤维细胞生长因子

Abstract:

Keywords:

基金项目(Foundation): 山东省医药卫生科研计划项目(2005-97)

作者(Author): 李斌;张伟;王健;孙水;吴帅;

Email:

DOI:

参考文献(References)：

• [1]Bernardo ME,Emons JA,Karperien M,et al.Human mesenchymal stem cells derived from bone marrowdisplay a better chondrogenic dif-ferentiation compared with other sources[J].Connect Tissue Res,2007,3:132-140.
• [2]Yoshimura H,Muneta T,Nimura A,et al.Comparison of rat mesen-chymal stem cells derived from bone marrow,synovium,periosteum,adipose tissue,and muscle[J].Cell Tissue Res,2007,3:449-462.
• [3]Kassem M,Kristiansen M,Abdallah BM.Mesenchymal stem cells:cell biology and potential use in therapy[J].Basic Clin Pharmacol Toxicol,2004,5:209-214.
• [4]吕昌伟,胡蕴玉,崔玉明,等.应力环境下三维诱导组织工程种植细胞修复关节软骨缺损[J].中国矫形外科杂志,2004,1:74-76.
• [5]Li WJ,Tuli RC,Okafor CK,et al.A three-dimensional nanofibrous scaffold for cartilage tissue engineering using human mesenchymal stem cells[J].Biomaterials,2005,6:599-609.
• [6]Barry F,Boynton RE,Liu B,et al.Chondrogenic differentiation of mes-enchymal stem cells from bone marrow:differentiation dependent gene expression of matrix components[J].Exp Cell Res,2001,2:189-200.
• [7]Fukumoto T,Sperling JW,Sanyal A,et al.Combined effects of insulin-like growth factor-Ⅰand transforming growth factor-beta1on periosteal mesenchymal cells during chondrogenesis in vitro[J].Oste-oarthritis Cartilage,2003,1:55-64.