戴闽;钟艳春;杨小刚;程明;杨康骅;程细高;

• 1:南昌大学第一附属医院骨二科

摘要(Abstract)：

[目的]研究植骨气囊模型体内血管内皮生长因子(VEGF)的表达及变化规律,探讨其在人工关节无菌性松动发生过程中的作用。[方法]建立人工关节无菌性松动小鼠植骨气囊模型,在实验组小鼠气囊内注入磨损颗粒悬浮液,对照组气囊内注入生理盐水,分别于植骨后1、2、3周分批处死小鼠,取囊壁连同颅骨片组织行HE染色观察囊壁炎症反应、骨片边缘溶解情况,半定量RT-PCR及免疫组织化学染色法观察VEGF基因及蛋白表达量的变化。[结果]接受磨损颗粒的实验组在各时间点VEGF基因表达量及阳性细胞率均明显高于对照组(P<0.05),其中以2周时表达量最高(P<0.05);且实验组炎症反应、骨片边缘溶解情况及微血管数量也相应地明显于对照组。[结论]磨损颗粒在体内可以诱导VEGF的表达,VEGF在假体周围微血管的生成、炎性细胞浸润及骨溶解中起重要作用。

关键词(KeyWords)： 磨损颗粒;血管内皮生长因子;人工关节;无菌性松动

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(编号:30760255)

作者(Author): 戴闽;钟艳春;杨小刚;程明;杨康骅;程细高;

Email:

DOI:

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