邱匀峰;吴小涛;赵梓汝;王运涛;

• 1:东南大学临床医学院
• 2:东南大学附属中大医院骨科

摘要(Abstract)：

[目的]建立成人退变髓核细胞的单层培养模型并观察其形态;测定其细胞周期,探讨传代后髓核细胞生长不佳的原因。[方法]取24例椎间盘突出症患者手术切除的椎间盘组织,分离出髓核组织,胰酶和胶原酶消化,DMEM/12培养基培养。倒置相差显微镜观察其形态学特征,流式细胞分析仪检测原代和P2细胞周期。[结果](1)原代髓核细胞生长良好,7 d后贴壁生长的细胞可达90%。(2)原代细胞凋亡率为(38.10±11.7)%,P2代凋亡率为(44.74±17.6)%,原代S期细胞(7.88±2.1)%,P2 S期细胞(2.76±0.7)%。[结论]传代后的髓核细胞凋亡率增高,S期细胞减少明显。

关键词(KeyWords)： 椎间盘;髓核细胞;细胞周期;凋亡

Abstract:

Keywords:

基金项目(Foundation): 东南大学国家自然基金预研项目资助(编号:XJ0690256)

作者(Author): 邱匀峰;吴小涛;赵梓汝;王运涛;

Email:

DOI:

参考文献(References)：

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