骨髓间充质细胞与速固化磷酸钙支架共培养的实验研究Experimental study on union culture of mesenchymal stem cell with fast setting calcium phosphate scaffold
王晶;张洹;刘华;
摘要(Abstract)：
[目的]观察人骨髓间充质干细胞在速固化磷酸钙骨水泥支架中的增殖和生长情况。[方法]将速固化壳聚糖-磷酸钙骨水泥支架材料预先成形。采用全骨髓法分离人骨髓间充质细胞并在体外对细胞进行培养和扩增。72h首次换液,细胞融合80%传代,取第3代细胞用于实验。将细胞与磷酸钙骨水泥支架复合培养,分别利用四甲基偶氮唑蓝MTT法及扫描电镜等检测细胞在材料中的增殖和生长情况。[结果]用酶标仪检测OD值提示共培养后,支架内的细胞数量逐渐增加。扫描电镜结果显示支架材料内部为多孔结构,孔内见有细胞生长,细胞表面可见分泌颗粒。[结论]速固化壳聚糖-磷酸钙骨水泥对人骨髓间充质干细胞有较好的细胞相容性,细胞可在材料内黏附和增殖,是理想的骨组织工程支架材料。
关键词(KeyWords)： 磷酸钙;壳聚糖;骨髓间充质细胞;组织工程;体外培养
基金项目(Foundation): 国务院侨办重点学科建设基金资助项目(2005)
作者(Author): 王晶;张洹;刘华;
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DOI:
参考文献(References)：
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