王业杨;李贵涛;王明森;李文俊;徐汪洋;周治来;张辉;

• 1:广东省第二人民医院骨科医学部
• 2:普宁市中医医院骨科

摘要(Abstract)：

[目的]探讨槲皮素(Quecetin,Que)对大鼠脊髓损伤保护作用及可能机制。[方法] SD大鼠60只,随机分为3组:假手术组(sham组),脊髓损伤组(SCI组),槲皮素治疗组(Que+SCI组)。采用改良的Allen's方法制备大鼠脊髓损伤模型。Que+SCI组大鼠术后每天给予20 mg/kg的槲皮素治疗,持续14 d。脊髓损伤后1、3、7和14 d采用BBB评分法评估各组大鼠后肢的运动功能。术后14 d,收集大鼠脊髓组织,HE染色法观察组织病理变化,蛋白印迹法评价槲皮素对TLR4/NF-KB信号通路的作用。炎症因子肿瘤坏死因子-α(TNF-α)和白介素-1β(IL-1β)的产生采用酶联免疫法检测。此外,通过蛋白印迹法测定凋亡相关蛋白cleaved-caspase 3、Bax和Bcl-2的表达水平。[结果]术后1、3、7和14 d,Que+SCI组BBB评分显著高于SCI组(P<0.05)。HE染色结果发现SCI组可见明显的空洞形成,空洞周围有胶质瘢痕增生,而在Que+SCI组上述病理改变显著减轻。Que+SCI组的TLR4/NF-κB信号通路蛋白、TNF-α和IL-1β,以及cleaved-caspase 3和Bax蛋白表达水平显著低于SCI组(P<0.05),而Que+SCI组的Bcl-2蛋白表达水平显著高于SCI组(P<0.05)。[结论]槲皮素抑制TLR4/NF-KB信号通路介导的炎症反应和细胞凋亡从而减轻脊髓损伤。

关键词(KeyWords)： 槲皮素;炎症反应;凋亡;TLR4/NF-κB信号通路

Abstract:

Keywords:

基金项目(Foundation): 广东省科技计划项目（编号：2012B031800266）;; 广东省第二人民医院青年科研基金项目（编号：YQ2017-011）;广东省第二人民医院博士工作站项目（编号：2019BSGZ005） 揭阳市科技计划项目（编号：2017YL031）

作者(Author): 王业杨;李贵涛;王明森;李文俊;徐汪洋;周治来;张辉;

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DOI:

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