不同分化程度的类成骨细胞对流体刺激的生物反应Biological responses of osteoblast-like cells in different maturation stages under shear flow
杜大江;高中礼;刘光耀;古川克子;牛田多加志;
摘要(Abstract)：
[目的]考察不同分化程度的骨组织细胞对流体刺激的生物反应。[方法]利用平行板流体刺激小室系统对以成骨分化诱导液诱导的不同分化程度类成骨细胞MC3T3-E1进行2 h流体刺激,剪切力为0.0 462 mN.s/cm2,24 h后收集样本,进行双链DNA和碱性磷酸酶测定。[结果]流体刺激24 h后,非诱导分化组ALP活性无明显变化,诱导分化4 d组ALP活性明显升高,诱导分化9 d组ALP活性明显降低。[结论]不同分化程度的类成骨细胞对流体刺激的反应不同,对骨的生长与重建有着重要意义。
关键词(KeyWords)： 机械刺激;成骨分化
基金项目(Foundation):
作者(Author): 杜大江;高中礼;刘光耀;古川克子;牛田多加志;
Email:
DOI:
参考文献(References)：
- (1)N illson BE,W estlin NE.Bone density in athletes[J].C lin O rthop,1917,77:179-182.
- (2)段小军,杨柳,何天佐.骨组织关键技术的研究进展[J].中国矫形外科杂志,2003,11(23):1628-1630.
- (3)Owan I,BurrDB,Turner CH,et al.M echanotransduction in bone:oste-ob lasts are more responsive to flu id forces than m echan ical strain[J].Am erican journal of Physiology-Cell Physiology,1997,42(3):810-815.
- (4)W einbaum S,Cow in SC,Zheng YA.A model for the excitation of os-teocytes by m echan ical load ing induced bone flu id shear stresses[J].JB iom ech,1994,27:339-360.
- (5)徐钢,陈鸿辉,杨小红,等.低强度超声促进兔骨-肌腱结合部早期恢复的初步观察[J].中国矫形外科杂志,2005,13(10):767-769.
- (6)Ibarak i K,Term ine JD,W h itson SW,et al.Bone matrix mRNA expres-sion in d ifferentiating fetal bovine osteob lasts[J].J Bone M iner Res,1992,7(7):743-754.
- (7)H illsley MA,Frangos JA.A lkaline phosphatase in osteoblasts is down-regulated by pulsatile flu id flow[J].Calcif Tissue Int,1997,60:48-53.