脊髓损伤相关中枢性疼痛与CGRP阳性的初级传入纤维再生的相关性研究Relationship between central pain following spinal cord injury and regeneration of CGRP- positive primary afferent fibers
林世德;张俊江;臧宇杰;赵廷宝;
摘要(Abstract):
[目的]观察脊髓损伤(spinal cord injury,SCI)后发生中枢性疼痛(central pain,CP)大鼠损伤远端脊髓背角中生长相关蛋白43(GAP-43)和降钙基因相关肽(CGRP)的共定位表达情况,探讨CP与CGRP阳性的初级传入神经纤维出芽再生之间的关系。[方法]SD大鼠16只,随机等分为SCI组和假术(SHAM)组。SCI组大鼠用NYU撞击器损伤L1节段脊髓,撞击力为10 g×12.5 mm。SHAM组大鼠仅切除椎板,不损伤脊髓。术后每天观察双后肢自噬现象,每周测量双后肢运动功能评分(BBB scores,developed by Basso,Beattie and Bresnahan at Ohio State University)。术后4~8周每周测量后肢触压痛痛阈及冷热过敏症状,了解CP的发生情况。术后8周每组取4只行损伤远端脊髓GAP-43与CGRP免疫荧光双标记并在共聚焦显微镜下观察二者的共定位表达情况。[结果]SCI组大鼠术后均出现双后肢瘫痪及明显的CP症状,其损伤远端脊髓背角中GAP-43与CGRP的共定位表达量明显升高,分布范围明显扩大,与SHAM组比较,差异有统计学意义(P<0.01)。[结论]CP的发生与SCI后感受伤害性刺激的CGRP阳性感觉传入纤维的出芽再生有关。
关键词(KeyWords): 脊髓损伤(SCI);中枢性疼痛(CP);生长相关蛋白43(GAP-43);降钙基因相关肽(CGRP)
基金项目(Foundation): 全军医学科技青年培育项目计划(项目编号:13QNP027)
作者(Author): 林世德;张俊江;臧宇杰;赵廷宝;
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DOI:
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