基于永生化表皮细胞工程化皮瓣体外形成的实验研究Experimental study of engineering flap formation based on immortalized keratinocytes in vitro
饶燕,方泽强,淡明斌,李德如
摘要(Abstract)：
目的:探讨永生化上皮细胞在工程化皮瓣中应用的可能性。方法:把人端粒酶催化亚基(hTERT)转染兔表皮细胞,筛选阳性克隆并在体外进行大量扩增;将扩增的永生化表皮细胞和平滑肌细胞与支架(PGA)复合并在体外培育后,植入裸鼠皮下;3～6个月取材,进行形态学和免疫组织化学评价。结果:实验组,术后6个月,复合体被新生的表皮和平滑肌组织所覆盖,抗人角细胞广谱角蛋白(AE1/AE3)抗体和抗人平滑肌a-肌动蛋白抗体染色呈强阳性,与对照组差别有显著性意义(P<0.01)。结论:永生化表皮细胞在皮瓣组织工程中具有较好的应用前景。
关键词(KeyWords)： 支架;永生化;组织工程;皮瓣
基金项目(Foundation):
作者(Author): 饶燕,方泽强,淡明斌,李德如
Email:
参考文献(References)：
- [1] Kuroyanagi Y. Regenerative medicine for skin [J]. Nippon Rinsho, 2003,61(3) :427～431．
- [2] Hori Y,Nakamura T,Kimura D,et al. Experimental study on tissue engineering of the small intestine by mesenchymal stem cell seeding [J]. J Surg Res,2002,102(2) :156～160．
- [3] Oshima H,Inoue H,Matsuzaki K,et al. Permanent restoration of human skin treated with cultured epithelium grafting-wound healing by stem cell based tissue engineering[J]. Hum Cell,2002,15(3) : 118～128．
- [4] Lai JY, Yoon CY,Yoo JJ,et al. Phenotypic and functional characterization of in vivo tissue engineered smooth muscle from normal and pathological bladders [J]. J Urol,2002,168(4) : 1853～1857．
- [5] Liu SQ,Moore MM,Yap C. Prevention of mechanical stretch-induced endothelial and smooth muscle eell injury in experimental vein grafts [J]. J Biomech Eng,2000,122(1) :31～38．
- [6] Thomas M,Yang L, Hornsby PJ. Formation of functional tissue from transplanted adrenocortical cells expressing telomerase reverse transcriptase [J]. Nat Biotechnol,2000,18(1) : 39～42．
- [7] Thomas M,Northrup SR,Hornsby PJ. Adrenocortical tissue formed by transplantation of normal clones of bovine adrenocortical cells in scid mice replaces the essential function of the animals adrenal glands [J]. Nat Meal,1997,3(9) :978～983．
- [8] Saxena AK,Willital GH,Vacanti JP. Vascularized three-dimensional skeletal muscle tissue-engineering [J]. Biomed Mater Eng, 2001,11(4) :275-281．
- [9] Germain L,Remy-Zolghadri M, Auger F. Tissue engineering of the vascular system: from capillaries to larger blood vessels [J]. Med Biol Eng Comput,2000,38(2) :232～240．