孙成良;刘秀美;刘莹;

• 1:山东大学附属省立医院骨科
• 2:山东大学齐鲁儿童医院病理科
• 3:山东大学附属省立医院病理科

摘要(Abstract)：

[目的]探讨人骨髓核分化抗原(MNDA)在骨肉瘤中的表达及临床意义。[方法]采用免疫组化法检测MNDA在20例骨软骨瘤和42例骨肉瘤组织中的表达,并分析MNDA表达程度与年龄、性别、病理类型和复发、转移等因素的关系。[结果]MNDA在骨肉瘤组的阳性表达率(17%)明显低于骨软骨瘤组(80%)(P<0.01)。MN-DA的阳性表达与性别、年龄无关(P>0.05),与骨肉瘤的病理类型及是否有复发转移相关。[结论]MNDA在骨肉瘤中低表达,为干扰素在骨肉瘤中的研究提供了理论依据。

关键词(KeyWords)： MNDA;骨肉瘤;免疫组织化学

Abstract:

Keywords:

基金项目(Foundation): 山东省自然科学基金ZR2009CM042

作者(Author): 孙成良;刘秀美;刘莹;

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DOI:

参考文献(References)：

• [1]Briggs RC,Shults KE,Flye LA,et al.Dysregulated human mye-loid nuclear differentiation antigen expression in myelodysplasticsyndromes:evidence for a role in apoptosis[J].Cancer Res,2006,66:4645-4651.
• [2]Doggett KL,Briggs JA,Linton MF,et al.Retroviral mediated ex-pression of the human myeloid nuclear antigen in a null cell line up-regulates Dlk1 expression[J].J Cell Bioch,2002,86:56-66.
• [3]龙汉安,张贤良.骨肉瘤分类和分子生物学研究进展[J].临床与实验病理学杂志,1999,4:336-338.
• [4]Briggs RC,Briggs JA,Ozer J,et al.The human myeloid cell nueleardifferentiation antigen gene is one of at least two related interferon-inducible genes located on chromosome lq that are expressed specif-ically in hematopoietic cells[J].Blood,1994,83:2153-2162.
• [5]Johnstone RW,Trapani JA.Transcription and growth regulatoryfunctions of the HIN-200 family of proteins[J].Mol Cell Biol,1999,19:5833-5838.
• [6]Staub E,Dahl E,Rosenthal A.The DAPIN family:a novel domainlinks apoptotic and interferon response proteins[J].Trends Bio-chem Sci,2001,26:83-85.
• [7]Inaba H,Glibettic M,Buck S,et al.Interferon-gamma sensitizesosteosarcoma cells to Fas-induced apoptosis by up-regulating Fasreceptors and caspase-8[J].Pediatr Blood Cancer,2004,43:729-736.
• [8]黄涛,吕刚,王岩峰,等.γ干扰素上调Fas表达诱导骨肉瘤细胞凋亡的研究[J].中国医科大学学报,2008,37:482-484.
• [9]张兰凤,肖学文,刘欣.干扰素抑制骨肉瘤机制的研究[J].四川解剖学杂志,2007,15:47-48.
• [10]Zhang Y,Howell RD,Alfonso1 DT,et al.IFI16 inhibits tumorige-nicity and cell proliferation of bone and cartilage tumor cells[J].Front Bios,2007,12:4855-4863.
• [11]Zhang Y,Howell RD,Alfonso DT,et al.IFI16 inhibits tumorige-nicity and cell proliferation of bone and cartilage tumor cells[J].Front Biosci,2007,9:4855-4863.
• [12]Kim MK,Mason JM,Li CA,et al.A pathologic link between wilmstumor suppressor gene,WT1,and IFI16[J].Neoplasia,2008,1:69-78.