孙明林;吕丹;朱雷;

• 1:武警医学院附属医院骨科

摘要(Abstract)：

[目的]观察不同代次正常软骨细胞和关节炎软骨细胞对琼脂糖-BMSCs向软骨细胞分化的促进作用。[方法]分离新西兰兔BMSCs,正常软骨细胞。制作兔关节炎模型,提取兔关节炎软骨细胞。将BMSCs和低熔点琼脂糖复合成凝胶块,放在自制的六孔板网格架上,构建兔软骨细胞-BMCSs共培养系统。在3、7、14 d取各组琼脂糖-BMSCs凝胶块进行实时定量PCR、GAG含量检测。[结果]兔关节炎模型制作成功,关节面色泽较灰暗,关节软骨粗糙。Normal P0-BMSCs组的II型胶原基因表达明显增强,Normal P3-BMSCs组Ⅰ、Ⅱ型胶原及蛋白聚糖基因表达均未见明显增强,OA P0-BMSCs组蛋白聚糖基因表达明显增强,OA P3-BMSCs组I型胶原基因表达水平在3个时间点均低于对照组。Normal P0-BMSCs组的GAG含量为5.7±0.49μg/mg(湿重),较对照组有明显上升。OA P0-BMSCs组GAG含量与对照组相比差异无统计学意义(P>0.05),其余三组与对照组相比差异均具有统计学意义(P<0.05)。[结论]兔正常P0软骨细胞与兔关节炎P0软骨细胞所分泌的形态发生素能够有效促进BMSCs向软骨细胞分化,而兔正常P3软骨细胞的促分化作用微弱,兔关节炎P3软骨细胞不能促进BMSCs向软骨细胞分化。

关键词(KeyWords)： 骨髓间充质干细胞;软骨细胞;共培养;形态发生素;软骨分化

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(NO10872147)

作者(Author): 孙明林;吕丹;朱雷;

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DOI:

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