BMP-2对人退变椎间盘髓核细胞影响的实验研究Effects of BMP-2 on human nucleus pulposus cells in the disc degeneration
梁伟国;叶冬平;李锋生;戴丽冰;沈雁;陈鸿辉;
摘要(Abstract):
[目的]探讨BMP-2对人退变髓核细胞合成细胞外基质的影响。[方法]分离、培养人退变椎间盘髓核细胞,取第2代髓核细胞,随机将退变椎间盘髓核细胞分为2组。A组:加入100 ng/ml BMP-2,B组:加入200 ng/mlBMP-2,C组:对照组,不加干扰因素。通过对试验组和对照组髓核细胞采用光镜、电镜等形态学方法进行大体形态和超微结构观察,细胞Ⅱ型胶原和糖胺多糖的mRNA表达。ELISA检测细胞培养上清中人Ⅱ型胶原含量,DMMB比色法检测细胞培养上清中糖胺多糖含量。[结果]髓核细胞中II型胶原、糖胺多糖表达水平实验组均高于对照组。[结论]BMP-2蛋白可促进退变腰椎间盘细胞分泌蛋白多糖和Ⅱ型胶原,增加细胞活性,恢复椎间盘的功能和活性,因此运用BMP-2椎间盘内注射有望成为椎间盘退变疾病生物治疗的方法之一。
关键词(KeyWords): 髓核细胞;Sox-9;BMP-2
基金项目(Foundation): 广州市医药卫生重点项目(编号:2009-zdi-04)资助;; 2010年广东省自然科学基金(项目编号:10151022001000005);2011年广东省自然科学基金(项目编号:S2011010000910)资助
作者(Author): 梁伟国;叶冬平;李锋生;戴丽冰;沈雁;陈鸿辉;
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参考文献(References):
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