siRNA特异性抑制骨肉瘤细胞株HER-2基因的表达Inhibition of HER-2 expression by RNA interference in osteosarcoma cells
王玉峰;王岩峰;祝勇;邴佩旭;
摘要(Abstract)：
[目的]观察RNA干扰技术对骨肉瘤细胞株(OS-732细胞)HER-2(表皮生长因子受体-2)表达的抑制效应。[方法]体外化学合成HER-2序列特异性双链RNA(siRNA),转染入OS-732骨肉瘤细胞株中,分别用RT-PCR、WesternBlot和免疫细胞化学法检测转染前后HER-2基因的mRNA和蛋白的表达情况,用MTT(四甲基偶氮唑盐比色法)检测细胞的增殖和化疗敏感性,观察细胞生物学特性的改变。[结果]HER-2siRNA对OS-732细胞HER-2mRNA表达明显抑制,作用约持续10d;干涉第9d,干涉组细胞蛋白表达减弱;细胞对顺铂的敏感性提高约10倍。[结论]体外合成的siRNA能有效抑制骨肉瘤OS-732细胞中HER-2基因的表达,抑制细胞的增殖,并提高了细胞对顺铂的敏感性。
关键词(KeyWords)： RNA干扰;siRNA;骨肉瘤;HER-2
基金项目(Foundation): 辽宁省自然科学基金资助项目(NO20052105)
作者(Author): 王玉峰;王岩峰;祝勇;邴佩旭;
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DOI:
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