张增山;文益民;

• 1:兰州军区兰州总医院脊柱外科

摘要(Abstract)：

[目的]探讨一定条件下骨髓间充质干细胞向神经元样细胞分化的能力,并进一步研究两种细胞联合移植治疗脊髓损伤(spinalcord injury,SCI)的效果。[方法]选取3个月龄雌性SD大鼠62只,2只处死后提取骨髓间充质干细胞用于细胞培养和分化研究,剩余大鼠采用改良Allen’s法制备T9～11SCI模型,然后随机分为4组(n=15),A组为空白对照组,B组为干细胞组,C组为神经元样细胞组,D组为联合移植组。诱导后的细胞采用免疫荧光方法检测细胞表面特异性标志,各组细胞用Hoechst33342标记,脊髓损伤模型建立1周后将细胞以局部注射的方法移植到损伤区,细胞移植后的1、2、4、6周对各组动物进行Basso-Beattie-Bresnahan(BBB)评分,并行HE染色、荧光显微镜、免疫荧光法检测细胞在体内的存活和分化。[结果]术后2、4、6周各细胞移植组BBB评分较空白对照组相比显著增加(P<0.05),D组高于B、C两组(P<0.05),B、C两组组间比较差异无统计学意义(P>0.05)。HE染色示B、C、D组术后6周的脊髓空洞较A组均减小,以D组最明显;荧光显微镜显示移植的细胞存活于脊髓损伤的区域;免疫荧光染色显示诱导后的细胞与体内存活的细胞表面特异性标志神经元特异性烯醇化酶(neuron specific enolase,NSE)、神经丝蛋白200(neuroflament200,NF-200)表达呈阳性,胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)表达呈弱阳性,其中D组NSE和NF-200阳性细胞数量较多,B、C两组较少。[结论]体内体外一定条件下骨髓间充质干细胞都有向神经元样细胞分化的能力;骨髓间充质干细胞和定向诱导的神经元样细胞都可用于移植治疗脊髓损伤,但以两者联合移植治疗的效果最佳。

关键词(KeyWords)： 骨髓间充质干细胞;神经元样细胞;细胞移植;脊髓损伤;细胞分化

Abstract:

Keywords:

基金项目(Foundation): 全军医学科学技术研究“十一五”计划课题资助项目(编号:06MA081)

作者(Author): 张增山;文益民;

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DOI:

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