张强;邵阳;赵昌松;高峥嵘;蔡娟;

• 1:首都医科大学附属北京地坛医院骨科

摘要(Abstract)：

[目的]探讨大鼠脊髓损伤后N-甲基-D-天门冬氨酸(NMDA)受体拮抗剂MK-801胚胎脊髓移植后对大鼠脊髓细胞凋亡的影响。[方法]将动物分为大鼠脊髓半切洞损伤后,分为应用胚胎脊髓和MK-801治疗组(A组),大鼠脊髓半切洞损伤应用胚胎脊髓移植组(B组),单纯脊髓半切洞损伤明胶海绵填充组(C组)。手术后1、3、7、14 d对脊髓损伤区进行细胞凋亡的检测(TUNEL),以及Bcl-2蛋白免疫反应表达的测定(免疫组化法)。采用计算机图像分析技术,进行定量分析。[结果]A、B、C三组中均发现凋亡细胞及BCL-2蛋白免疫反应阳性细胞,图像分析发现,细胞凋亡率为:C>B>A,Bcl-2免疫反应阳性细胞表达顺序为:A>B>C。[结论]大鼠脊髓损伤后应用NMDA受体拮抗剂MK-801和胚胎脊髓移植能抑制脊髓损伤后细胞凋亡。

关键词(KeyWords)： 脊髓损伤;NMDA受体;MK-801;胚胎脊髓移植;凋亡

Abstract:

Keywords:

基金项目(Foundation): The National Natural Science Foundation of China,No.30300075;; Postdoctoral Science Foundation,No.20080440995;; Sichuan Science Fund for Outstanding Youths,No.05ZQ026-020

作者(Author): 张强;邵阳;赵昌松;高峥嵘;蔡娟;

Email:

DOI:

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