尹国栋;汤逊;林月秋;徐永清;周田华;

• 1:成都军区昆明总医院全军骨科中心
• 2:成都军区昆明总医院全军骨科中心
• 3:成都军区昆明总医院全军骨科中心
• 4:成都军区昆明总医院全军骨科中心

摘要(Abstract)：

[目的]探索人胚胎嗅鞘细胞(hOECs)移植修复大鼠脊髓全横断损伤的可行性。[方法]分离、培养并鉴定人胚嗅鞘细胞,24只W istar大鼠,随机分为实验组和对照组,均行T10节段脊髓全横断。术后第9~10 d,实验组、对照组分别移植Hoechst33342标记的hOECs 5μl(2.5×105个细胞)、DMEM-F12培养基5μl。移植术后第1、2、4、6、8、10周进行BBB运动功能评分,取材行荧光化学(Hoechst33342)和免疫组织化学染色(P75、NF-200、Syn-aptophysin)。双盲条件下进行数据统计。[结果]移植的hOECs可以在损伤脊髓内存活10周以上,可向损伤脊髓头尾两端迁移;术后4~10周实验组的BBB运动功能评分明显高于对照组(P<0.01);P75染色实验组呈阳性反应;NF-200和Synaptophysin免疫组化染色,实验组神经纤维、突触数目和密度都较对照组高。[结论]hOECs移植对脊髓全横断损伤大鼠运动功能恢复具有促进作用。

关键词(KeyWords)： 脊髓损伤;人胚胎嗅鞘细胞;细胞移植;BBB评分

Abstract:

Keywords:

基金项目(Foundation): 云南省自然科学基金资助项目(2002C0070M)

作者(Author): 尹国栋;汤逊;林月秋;徐永清;周田华;

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DOI:

参考文献(References)：

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