于磊;宋红星;胡勇;

• 1:北京世纪坛医院脊柱外科
• 2:首都医科大学研究生院
• 3:香港大学李嘉诚医学院

摘要(Abstract)：

[目的]采用聚氨酯构建慢性颈脊髓压迫动物模型,观察受压颈脊髓内早期炎症反应。[方法] SD大鼠随机均分为颈髓压迫模型(CSM)组和对照组。CSM)组C6椎板下置入新型吸水性可膨胀生物材料聚氨酯,对照组不置入可膨胀性生物材料。建模14 d后行颈椎核磁共振检查,观察大鼠脊髓受压影像学表现。建模3、7、14 d后分别取大鼠受压颈脊髓组织,采用蛋白印迹方法检测受压颈脊髓组织p-MK2、炎症因子(NF-κB)、凋亡相关蛋白(caspase-8、Bax/Bcl-2)的变化。[结果]大鼠颈椎MRI检查提示CSM组矢状位、轴位MRI可见脊髓明显受压、局部变细。CSM组SD大鼠p-MK2、NF-κB、caspase-8和Bax表达较对照组显著增多(P<0.05),而Bcl-2的表达则显著降低(P<0.05)。[结论]聚氨酯构建的动物模型中受压的颈脊髓内早期出现了炎症反应,MK2可能参与了炎症反应。

关键词(KeyWords)： 慢性脊髓压迫;动物模型;信号通路;炎症因子;凋亡蛋白

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 于磊;宋红星;胡勇;

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参考文献(References)：

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