王俊义;席光辉;张成勇;薛龙海;朱书涛;刘洋;

• 1:河南大学淮河医院骨科

摘要(Abstract)：

[目的]观察miR-100对不同骨肉瘤细胞系的表达差异,研究miR-100对骨肉瘤细胞MG-63增殖、侵袭迁移能力的影响。[方法]采用qRT-PCR检测miR-100在骨肉瘤细胞系中的表达差异。通过脂质体2000转染说明对骨肉瘤细胞MG-63进行转染操作,分别构建miR-100过表达和miR-100阴性对照组。转染骨肉瘤成功后,通过CCK-8实验检测miR-100对骨肉瘤细胞增殖能力的影响,同时Transwell实验检测骨肉瘤细胞侵袭、迁移能力变化。[结果]miR-100在骨肉瘤细胞系中表达差异显著(P<0.05),且差异具有统计学意义;相比miR-100NC组,过表达miR-100增殖、侵袭、迁移能力明显下降(P<0.05),且差异具有统计学意义。[结论]miR-100在骨肉瘤中呈现低表达;过表达miR-100抑制骨肉瘤细胞增殖、侵袭、迁移能力。miR-100可能会成为未来骨肉瘤治疗的潜在靶点。

关键词(KeyWords)： 骨肉瘤;miR-100;增殖;侵袭;迁移

Abstract:

Keywords:

基金项目(Foundation): 河南省教育厅科学技术研究重点项目(编号:15A320046)

作者(Author): 王俊义;席光辉;张成勇;薛龙海;朱书涛;刘洋;

Email:

DOI:

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