骨髓基质细胞体外培养骨发生潜能及条件研究Osteogenic Potential of the Cultured Bone Marrow Stem Cell(BMSc) in Vitro
金丹,裴国献,王前,魏宽海
摘要(Abstract)：
目的 :建立一种骨髓基质细胞 (BMSc)向成骨细胞转化的体外培养方法 ,观察其成骨过程 ,并探讨部分因子在BMSc转化及增殖中的作用。方法 :将兔BMSc悬液进行体外培养 ,进行形态学观察和组织学检测 ;检测bFGF对BM Sc的促增殖作用。结果 :传代培养的细胞ALP阳性率达 80 %以上 ,细胞逐渐分化成散在致密的岛状结构并分泌细胞外基质形成钙结节 ;bFGF能刺激BMSc的增殖。结论 :培养的BMSc在体外仍具有成骨能力。地塞米松、β -甘油磷酸钠和维生素C可促进BMSc分化为成骨细胞 ,bFGF可促进BMSc的增殖
关键词(KeyWords)： 骨髓基质细胞;成骨作用;组织工程
基金项目(Foundation):
作者(Author): 金丹,裴国献,王前,魏宽海
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