建立成骨与破骨细胞共育体系的一种方法A Method for Development of a Co-culture System:Osteoblast and Osteoclast Interaction
史风雷,王海彬,杨勇,樊粤光,袁浩
摘要(Abstract)：
目的:建立成骨细胞和破骨细胞共育的体外模型,为研究骨生理和病理提供新的方法。方法:从胎鼠和4周鼠中分离成骨和破骨细胞,并按照10:1、100:1的比例混合培养,应用碱性磷酸酶(AKP)、抗酒石酸(TRAP)、甲苯胺蓝染色法,鉴定成骨及破骨细胞,并测量碱性磷酸酶的含量,从而建立一个较稳定的共育体系。结果:破骨细胞和成骨细胞达到了相互接触培养,且随着破骨细胞数量的不同,碱性磷酸酶的含量会有不同的变化。结论:该模型可用骨形成和吸收代谢的相关研究。
关键词(KeyWords)： 成骨细胞;破骨细胞;共育体系
基金项目(Foundation): 中国博士后基金(200031);; 国家自然基金(30100244);; 广东省自然基金(020778)
作者(Author): 史风雷,王海彬,杨勇,樊粤光,袁浩
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参考文献(References)：
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