NS-398对骨肉瘤细胞的作用及其对Survivin表达的影响The influence of NS-398 on induction of apoptosis and expression of survivin in osteosarcoma cells
梁新军,夏仁云,俞猛,夏侃
摘要(Abstract)：
目的:观察选择性环氧合酶-2抑制剂NS-398对人骨肉瘤细胞系MG-63的凋亡的影响及其对survivin基因表达的影响。方法:体外培养骨肉瘤细胞系MG-63,应用MTT法研究不同浓度NS-398对骨肉瘤细胞系MG-63的凋亡诱导作用,流式细胞仪检测细胞周期的改变及凋亡率的变化,RT-PCR法分析NS-398对骨肉瘤细胞系MG-63中sur-vivin基因表达的影响。结果:NS-398可诱导骨肉瘤细胞系MG-63发生凋亡,并具有剂量依赖性,在NS-398浓度为200μmol/L时,对细胞生长有明显的抑制作用。流式细胞仪检测显示NS-398引起MG-63细胞凋亡率增加。RT-PCR分析显示经NS-398作用后的骨肉瘤细胞系MG-63表达survivin较用药前明显下降。结论:NS-398对骨肉瘤细胞系MG-63有杀伤作用,其作用可能通过抑制survivin的表达,促进肿瘤细胞的凋亡而实现。
关键词(KeyWords)： NS-398;骨肉瘤;Survivin
基金项目(Foundation):
作者(Author): 梁新军,夏仁云,俞猛,夏侃
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参考文献(References)：
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